Article
Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model
Registro en:
TELES, Amanda Mara et al. Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model. Marine Drugs, v. 18, n. 11, 541, p. 1-19, Oct. 2020.
1660-3397
10.3390/md18110541
Autor
Teles, Amanda Mara
Pontes, Leticia Prince Pereira
Guimarães, Sulayne Janayna Araújo
Butarelli, Ana Luiza
Silva, Gabriel Xavier
Nascimento, Flavia Raquel Fernandes do
Bezerra, Guesa Felipa de Barros
Moragas-Teles, Carla Junqueira
Costa, Rui Miguel Gil da
Silva, Marcos Antonio Custodio Neto da
Souza, Fernando Almeida
Calabrese, Kátia da Silva
Santos, Ana Paula Silva Azevedo
Nascimento, Maria do Desterro Soares Brandão
Resumen
Background: This study addresses the antitumoral properties of Penicillium purpurogenum isolated from a polluted lagoon in Northeastern Brazil. Methods: Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor model was used for antitumor activity. Female Swiss mice were divided into groups (n = 10/group) as follows: The negative control (CTL−), treated with a phosphate buffered solution; the positive control (CTL+), treated with cyclophosphamide (25 mg/kg); extract treatments at doses of 4, 20, and 100 mg/kg; animals without tumors or treatments (Sham); and animals without tumors treated with an intermediate dose (EAE20). All treatments were performed intraperitoneally, daily, for 15 days. Subsequently, the animals were euthanized, and the tumor, lymphoid organs, and serum were used for immunological, histological, and biochemical parameter evaluations. Results: The extract was rich in meroterpenoids. All doses significantly reduced tumor size, and the 20 and 100 mg/kg doses reduced tumor-associated inflammation and tumor necrosis. The extract also reduced the cellular infiltration of lymphoid organs and circulating TNF-α levels. The extract did not induce weight loss or renal and hepatic toxic changes. Conclusions: These results indicate that P. purpurogenum exhibits immunomodulatory and antitumor properties in vivo. Thus, fungal fermentation is a valid biotechnological approach to the production of antitumor agents.