Article
Antimicrobial Peptides from Adenanthera pavonina L. Seeds: Characterization and Antifungal Activity
Registro en:
SOARES, Julia Ribeiro; et al. Antimicrobial Peptides from Adenanthera pavonina L. Seeds: Characterization and Antifungal Activity. Protein & Peptide Letters, v.19, p.520-529, 2012.
0929-8665
1875-5305
Autor
Soares, Julia Ribeiro
Carvalho, André de Oliveira
Santos, Izabela Silva dos
Machado, Olga Lima Tavares
Nascimento, Viviane V.
Vasconcelos, Ilka Maria
Ferreira, André Teixeira da Silva
Perales, Jonas Enrique de Aguilar
Gomes, Valdirene Moreira
Resumen
In this study, the antifungal activity of peptides extracted from Adenanthera pavonina seeds was assessed. Peptides
were extracted and fractionated by DEAE-Sepharose chromatography. The non-retained D1 fraction efficiently inhibited
the growth of the pathogenic fungi. This fraction was later further fractionated by reversed-phase chromatography,
resulting in 23 sub-fractions. All separation processes were monitored by tricine-SDS-PAGE. Fractions H11 and H22
strongly inhibited the growth of Saccharomyces cerevisiae and Candida albicans. Fraction H11 caused 100% death in S.
cerevisiae in an antimicrobial assay. The complete amino acid sequence of the peptide in fraction P2 was determined, revealing
homology to plant defensins, which was named ApDef1. Peptides from fraction H22 were also sequenced. 2030-01-01