Article
Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene
Registro en:
SANTOS, Eduardo L. et al. Enhanced antibacterial effect of antibiotics by the essential oil of Aloysia gratissima (Gillies & Hook.) Tronc. and its major constituent beta-caryophyllene. Phytomedicine Plus, 2021.
2667-0313
10.1016/j.phyplu.2021.100100
Autor
Santos, Eduardo L.
Freitas, Priscilla R.
Araújo, Ana Carolina J.
Almeida, Ray S.
Tintino, Saulo R.
Paulo, Cicera Laura R.
Silva, Ana Cristina A.
Silva, Luiz E.
Amaral, Wanderlei do
Deschamps, Cícero
Siqueira Junior, José Pinto
Barbosa Filho, José Maria
Sousa, Gabriela Ribeiro de
Ribeiro Filho, Jaime
Coutinho, Henrique D. M.
Resumen
a Department of Biological Chemistry, Regional University of Cariri - URCA, Crato, Brazil b Maurício de Nassau College -UNINASSAU, Petrolina, PE, Brazil c Post graduate Program in Sustainable Territorial Development, Federal University of Parana ´ – UFPR, Matinhos, Brazil d Post graduate Program in Agronomy, Federal University of Parana ´ - UFPR, Curitiba, Brazil e Department of Molecular Biology, Federal University of Paraiba-UFPB, Joao ˜ Pessoa 58051-900, PB, Brazil f Departmen of Pharmacy, Federal University of Paraiba-UFPB, Joao ˜ Pessoa 58051-900, PB, Brazil g Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador, BA, Brazil Aloysia gratissima (Verbenaceae), popularly known as bee-brush or whitebrush, has been widely
used in Brazilian folk medicine as analgesic, expectorant and antimicrobial. Phytochemical studies have identified
β-caryophyllene as one of the major components of the essential oil of this plant. This bioactive sesquiterpene
has been shown to have anti-inflammatory and antibacterial activities.
Purpose: The present study aimed to characterize the chemical profile and evaluate the antibacterial and
antibiotic-enhancing activities of the essential oil obtained from Aloysia gratíssima (EOAG) and β-caryophyllene.
Methods: The phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GCMS).
The antibacterial activity against Pseudomonas aeruginosa 24, Staphylococcus aureus 10, and Escherichia coli
06 was assessed by determining the minimum inhibitory concentration (MIC) using the broth microdilution
method. The potentiation of the antibiotic activity by the EOAG and the β-caryophyllene was performed using
the MIC evaluation of the antibiotic alone or in association with the essential oil and its main compound. To
evaluate the minimum reduction of the drug concentration necessary, the Dose Reduction Index (DRI) was
calculated.
Results: A reduction in the MIC of the antibiotics against strains treated simultaneously with the essential oil or
β-caryophyllene was observed. The GC-MS analysis of the EOAG identified 30 compounds, including β-caryophyllene
as the major component. Both OEAG and β-caryophyllene presented antibacterial activity against
S. aureus, in addition to potentiating the action of norfloxacin against S. aureus, P. aeruginosa, and E. coli.
Conclusions: These substances also reversed the antibiotic resistance to gentamicin and erythromycin against of
S. aureus and P. aeruginosa, respectively.