Article
Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source‑Tracking Markers
Registro en:
SANTOS, Debora Regina Lopes dos Santos et al. Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source‑Tracking Markers. Food and Environmental Virology, v. 15. p. 21-31, 2023.
1867-0334
10.1007/s12560-022-09544-x
Autor
Santos, Debora Regina Lopes dos
Sales, Marcelle Silva
Fumian, Tulio Machado
Maranhão, Adriana Gonçalves
Malta, Fábio Correia
Ferreira, Fernando César
Pimenta, Marcia Maria
Miagostovich, Marize Pereira
Resumen
This study assessed the sources of contamination of water matrices in a rural area using detection of a host-specifc virus
(human adenovirus [HAdV], porcine adenovirus [PAdV] and bovine polyomaviruses [BoPyV]) as potential microbial sourcetracking tool, and rotavirus A [RVA], given its epidemiological importance in Brazil. From July 2017 to June 2018, 92
samples were collected from eight points (P1-P8) of surface and raw waters in southeastern region of Brazil. Fifty-fve
(59.8%) were positive for HAdV, 41 (44.5%) for RVA, 10 (10.9%) for PAdV and four (4.3%) for BoPyV. HAdV and RVA
were detected at all sites, and over the entire sampling period, PAdV was detected at a porcine breeding area and at Guarda
River site, presenting high concentrations up to 2.6× 109
genome copies per liter [GC/L], and viral concentrations ranging
from 9.6× 101
to 7.1× 107
, while BoPyV (1.5× 104
GC/L–9.2× 105
GC/L) was only detected in samples from the bovine
breeding areas. The combination of human and animal virus circulation presents a potential impact in the environment due
to raw sewage discharge from regional communities, as well as potential hazard to human and animal health. 2028