Article
In cellulo and in vivo assays for compound testing against Trypanosoma cruzi
Registro en:
GUERRA-SLOMPO, Eloise Pavão et al. In cellulo and in vivo assays for compound testing against Trypanosoma cruzi. STAR Protocols, v. 4, n. 102058, p. 1–42, 2023.
2666-1667
10.1016/j.xpro.2023.102058
Autor
Guerra- Slompo, Eloise Pavão
Picchi-Constante, Gisele Fernanda Assine
Marek, Martin
Romier, Christophe
Sippl, Wolfgang
Zanchin, Nilson Ivo Tonin
Resumen
Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determination. Compounds showing high SI are tested using in vivo infection models in parallel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment.