Article
Virome in roof-harvested rainwater of a densely urbanized low-income region
Registro en:
PRADO, Tatiana et al. Virome in roof-harvested rainwater of a densely urbanized low-income region. Science of the Total Environment. v. 807, 15078, p. 1 - 8, 2022.
0048-9697
10.1016/j.scitotenv.2021.150778
Autor
Prado, Tatiana
Shubo, Tatsuo
Freitas, Lucas
Leomil, Luciana
Maranhão, Adriana Gonçalves
Miagostovich, Marize Pereira
Resumen
Rainwater harvesting has been considered an affordable practice to supplement the conventional sources of
water supply for potable and non-potable uses worldwide. This study characterizes the viral community found
in roof-harvested rainwater (RHRW) samples obtained under different rain volumes in a densely urbanized
low-income region in Rio de Janeiro, Brazil. Three pilot-scale standardized metal-sheet roofs (same catchment
area, material age, and slope – 3%) were installed in the study area aiming at obtaining more reliable and representative
samples. Fifty-four samples were collected fromsix rainfall events fromJanuary to April 2019 and concentrated
by the skimmed-milk flocculation method. Pools of different rainfall volumes were submitted to high
throughput sequencing using the shotgunmetagenomic approach. Sequencing was performed on NextSeq platform.
Genomic analysis of the virus community revealed that most are RNA non-human viruses, including two
main families: Dicistroviridae and Iflaviridae, recognized for infecting arthropods. Bacteriophages were also relatively
abundant, with a predominance of DNA phages belonging to Microviridae and Siphoviridae families, showing
percentages from5.3 and 3.7% of the total viral hits present in these samples, respectively. Viral genomic RNA
viruses (77%) predominated over DNA viruses (23%). Concerning number of viral species identified, a higher percentage
was observed for plant viruses (12 families, 58%). Hepatitis A virus and human klassevirus 1 were detected
among the established human pathogens, suggesting the need for RHRW treatment before it is considered for human consumption. Australian bat lyssavirus was also detected, emphasizing the importance of
environmentalmonitoring facing emerging viruses. The results corroborate the influence of the surrounding area on the rainwater quality. 2023