Article
Changes in amounts of total salivary gland proteins of Lutzomyia longipallpis (Diptera: Psychodidae) according to age and diet
Registro en:
PRATES, D. B. et al. Changes in amounts of total salivary gland proteins of Lutzomyia longipallpis (Diptera: Psychodidae) according to age and diet. Journal of Medical Entomology, v. 45, n. 3, p. 409-413, 2008.
0022-2585
Autor
Prates, Deboraci Brito
Santos, L. D.
Miranda, José Carlos
Souza, A. P. A.
Palma, Mário Sérgio
Barral Netto, Manoel
Barral, Aldina Maria Prado
Resumen
Saliva plays important roles in facilitation of a bloodmeal, lubrication of mouthparts, and
parasite transmission for some vector insects. Salivary composition changes during the lifetime of an
insect, and differences in the salivary proÞle may inßuence its functions. In this report, the amount
and proÞle of salivary gland protein of the American visceral leishmaniasis vector Lutzomyia longipalpis
(Lutz & Neiva, 1912) were analyzed at different times of insect development and diet. Protein
content from unfed female sand ßies increased signiÞcantly with age, and a signiÞcant difference was
observed in sugar-fed females during the Þrst 10 d of adult life. Salivary protein content sharply
decreased 1 d after blood feeding, with gradual increase in concentration the following days. SDSpolyacrylamide
gel electrophoresis analysis revealed that most polypeptides present in the saliva of
sugar-fed also were present in the saliva of blood-fed females. Understanding changes in sand ßyÕs
saliva contents at distinct days after emergence and the inßuence of a bloodmeal in this aspect may
reveal the role played by saliva during leishmaniasis transmission.