This survey examined 30 samples of groundnut and 15 groundnut products from three states (Khartoum, Kordofan and Gadarif) of Sudan for AFB1, AFB2, AFG1, and AFG2 using high performance liquid chromatography (HPLC) method with fluorescence detection. The frequency of contaminated groundnut samples with AFB1 from Khartoum, Gadarif and Kordofan state was 58.3%, 57.1%, and 66.7%, respectively. No sample of groundnut or groundnut product was contaminated with AFG1 or AFG2. The limit of detections (LODs) and limit of quantifications (LOQs) were found to be in a range between 0.01-0.02 ng g-1 and 0.03-0.05 ng g-1, respectively. Some groundnut samples contained AFB1 concentrations above the EU regulatory limits. The highest contaminated samples with aflatoxins were found in Kordofan state.