Aims: This work aimed to assess how seed size, vials, vial sealing (in vitro), and substrate (in vivo) affect C. regium germination and emergence. This study shall contribute to the viable production of C. regium seedlings. Study Design: The experimental design used in these experiments was randomized. Place and Duration of Study: Department of Plant Biotechnology, Universidade de RibeirãoPreto, between March 2010 and December 2010. Methodology: This work has evaluated how seed size, vials, vial sealing (in vitro), and substrate (in vivo) influence the germination and emergence of C. regium. Results: The results showed that cultivation of C. regium seedlings from seeds is viable, irrespective of seed size. Vial oxygenation is an important parameter to consider in vitro, to obtain a larger number of normal seedlings. As for in vivo conditions, germination should be conducted in sand, to ensure a greater amount of young seedlings. Conclusion: The results presented here attested that it is possible to produce C. regium seedlings from seeds of any size both in vivo and in vitro conditions. In vitro, it is important to consider vial oxygenation, in order to obtain a greater amount of normal seedlings. In vivo, germination should be conducted in sand, to ensure production of a large quantity of seedlings.Department of Plant Production, Faculty of Agricultural Sciences, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilDepartment of Organic Chemistry, Core Bioassays, Biosynthesis and Ecophysiology of Natural Products, Institute of Chemistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Plant Biotechnology, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil.Department of Plant Production, Faculty of Agricultural Sciences, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilDepartment of Organic Chemistry, Core Bioassays, Biosynthesis and Ecophysiology of Natural Products, Institute of Chemistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, Brazil