Artigo
The role of nicotine, cotinine and caffeine on the electrochemical behavior and bacterial colonization to cp-Ti
Registro en:
Materials Science & Engineering. C, Materials For Biological Applications, v. 56, p. 114-124, 2015.
1873-0191
10.1016/j.msec.2015.06.026
26249572
Autor
Barão, Valentim A. R.
Ricomini-Filho, Antonio P.
Faverani, Leonardo P. [UNESP]
Del Bel Cury, Altair A.
Sukotjo, Cortino
Monteiro, Douglas R. [UNESP]
Yuan, Judy Chia-Chun
Mathew, Mathew T.
Amaral, Regiane C. do
Mesquita, Marcelo F.
Silva, Wander J. da
Assunção, Wirley G. [UNESP]
Resumen
Although smoking promotes deleterious effect to bone healing, there is a lack of study investigating its role on the implant structure and biofilm growth. We hypothesized that nicotine, cotinine and caffeine would impair the corrosion resistance of commercially-pure titanium (cp-Ti) and would enhance Streptococcus sanguinis biofilm growth. Neither the smoking products nor the caffeine affected the corrosion tendency (P>.05) and the oxide layer resistance (P=.762) of cp-Ti. Lower capacitance values were noted in the presence of nicotine (P=.001) and cotinine (P=.0006). SEM showed no pitting corrosion, and the EDS spectra did not differ among groups. Nicotine (300μg/mL) induced higher surface roughness (P=.03) and greater surface change of cp-Ti. Nicotine at 3μg/mL, and cotinine at 0.3 and 3μg/mL increased the number of viable cells (P<.05). Biofilm exposed to nicotine (0.3, 3 and 30μg/mL) (P=.025, .030, .040, respectively) and cotinine (3 and 30μg/mL) (P=.027, .049, respectively) enhanced carbohydrate content. Biofilm biomass and protein content were similar among groups (P>.05). These findings suggest a greater biofilm accumulation in smokers, a risk factor that may lead to peri-implantitis. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo, Brazil, 13414-903. Department of Physiological Science, Piracicaba Dental School, University of Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo, Brazil, 13414-903. Department of Dental Materials and Prosthodontics, Aracatuba Dental School, Univ Estadual Paulista (UNESP), José Bonifácio, 1193, Araçatuba, São Paulo, Brazil, 16015-050. Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo, Brazil, 13414-903. Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, 801S Paulina, Chicago, IL, USA, 60612. Department of Orthopedic Surgery, Rush University Medical Center, 1611W Harrison, Chicago, IL, USA, 60612. Department of Public Health, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil. Department of Dental Materials and Prosthodontics, Aracatuba Dental School, Univ Estadual Paulista (UNESP), José Bonifácio, 1193, Araçatuba, São Paulo, Brazil, 16015-050. FAPESP: 2011/20017-0 FAPESP: 2011/20021-7