Detection of CD4+ and CD8+ lymphocytes in the intestine of broiler chicks treated with lactobacillus spp. and challenged with Salmonella enterica serovar enteritidis

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.creatorNoujaim, J. C. [UNESP]
dc.creatorAndreatti Filho, Raphael Lucio [UNESP]
dc.creatorLima, E. T. de [UNESP]
dc.creatorOkamoto, Adriano Sakai [UNESP]
dc.date2014-05-27T11:23:56Z
dc.date2014-05-27T11:23:56Z
dc.date2009-07-01
dc.date.accessioned2023-09-12T07:20:26Z
dc.date.available2023-09-12T07:20:26Z
dc.identifierhttp://dx.doi.org/10.1590/S1516-635X2009000300008
dc.identifierRevista Brasileira de Ciencia Avicola, v. 11, n. 3, p. 187-193, 2009.
dc.identifier1516-635X
dc.identifierhttp://hdl.handle.net/11449/130633
dc.identifier10.1590/S1516-635X2009000300008
dc.identifierS1516-635X2009000300008
dc.identifierWOS:000272254000008
dc.identifier2-s2.0-72249115637
dc.identifierS1516-635X2009000300008-en.pdf
dc.identifier8502462873517464
dc.identifier4703983380224373
dc.identifier0000-0002-0355-9841
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8779937
dc.descriptionThe expression of immune response as a leukocytic infiltrate by CD4+ and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks fed Lactobacillus spp or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis (SE) was studied using immunohistochemistry. Three hundred and twenty day-of-hatch broiler chicks were divided into four groups of 80 birds each and orally received L. reuteri, L. salivarius, L. acidophilus, or CM. Each group was subdivided into four subgroups of 20 birds each, classified as follows: a subgroup did not receive any oral treatment (negative control), subgroup treated with L. spp or CM, subgroup treated with L. spp or CM and challenged with SE, and subgroup only challenged with SE (positive control). The results show that the oral treatment with L. reuteri, L. salivarius, L. acidophilus, or CM and challenge or not with SE stimulated bird immune response as determined by the leukocytic infiltrate by CD8+ lymphocytes followed by CD4+ in the epithelium and in the lamina propria of the duodenum, jejunum, and cecum of chicks up to 12 days of age. CD8+ lymphocyte number was significantly higher in the intestine of chicks receiving CM and challenged with SE. The duodenum, followed by the jejunum, were the segments in which the immune response, as shown by T, CD4+ and CD8+ cells, was stimulated with the greatest intensity.
dc.descriptionSão Paulo State Univ, Coll Vet Med & Anim Sci, Dept Clin Vet Med, UNESP,FMVZ, BR-18618000 Botucatu, SP, Brazil
dc.descriptionSão Paulo State Univ, Coll Vet Med & Anim Sci, Dept Clin Vet Med, UNESP,FMVZ, BR-18618000 Botucatu, SP, Brazil
dc.format187-193
dc.languageeng
dc.publisherFacta-fundacio Arnco Ciência Tecnologia Avicolas
dc.relationRevista Brasileira de Ciência Avícola
dc.relation0.463
dc.rightsAcesso aberto
dc.sourceScopus
dc.subjectChicken
dc.subjectImmunohistochemistry
dc.subjectLymphocytes CD4+ and CD8+
dc.subjectProbiotic
dc.subjectSalmonella
dc.subjectAves
dc.subjectLactobacillus
dc.subjectLactobacillus acidophilus
dc.subjectLactobacillus reuteri
dc.subjectLactobacillus salivarius
dc.subjectSalmonella enterica
dc.subjectSalmonella enteritidis
dc.titleDetection of CD4+ and CD8+ lymphocytes in the intestine of broiler chicks treated with lactobacillus spp. and challenged with Salmonella enterica serovar enteritidis
dc.typeArtigo


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