Artigo
Development of lamellar gel phase emulsion containing marigold oil (Calendula officinalis) as a potential modern wound dressing
Registro en:
European Journal Of Pharmaceutical Sciences. Amsterdam: Elsevier Science Bv, v. 71, p. 62-72, 2015.
0928-0987
10.1016/j.ejps.2015.01.016
WOS:000351807400007
4842462513285606
Autor
Okuma, Cindy Hana
Andrade, Thiago Antônio Moretti de
Caetano, Guilherme Ferreira
Finci, Lorenzo I.
Maciel, Naira Rezende
Topan, José Fernando
Cefali, Letícia Caramori [UNESP]
Polizello, Ana Cristina Morseli
Carlo, T.
Rogerio, A. P.
Spadaro, Augusto Cesar Cropanese
Isaac, Vera Lucia Borges [UNESP]
Frade, Marco Andrey Cipriani
Rocha-Filho, Pedro Alves da
Resumen
Appropriate therapeutics for wound treatments can be achieved by studying the pathophysiology of tissue repair. Here we develop formulations of lamellar gel phase (LGP) emulsions containing marigold (Calendula officinalis) oil, evaluating their stability and activity on experimental wound healing in rats. LGP emulsions were developed and evaluated based on a phase ternary diagram to select the best LGP emulsion, having a good amount of anisotropic structure and stability. The selected LGP formulation was analyzed according to the intrinsic and accelerated physical stability at different temperatures. In addition, in vitro and in vivo studies were carried out on wound healing rats as a model. The LGP emulsion (15.0% marigold oil; 10.0% of blend surfactants and 75.0% of purified water [w/w/w]) demonstrated good stability and high viscosity, suggesting longer contact of the formulation with the wound. No cytotoxic activity (50-1000 mu g/mL) was observed in marigold oil. In the wound healing rat model, the LGP (15 mg/mL) showed an increase in the leukocyte recruitment to the wound at least on days 2 and 7, but reduced leukocyte recruitment after 14 and 21 days, as compared to the control. Additionally, collagen production was reduced in the LGP emulsion on days 2 and 7 and further accelerated the process of re-epithelialization of the wound itself. The methodology utilized in the present study has produced a potentially useful formulation for a stable LGP emulsion-containing marigold, which was able to improve the wound healing process. (C) 2015 Elsevier B.V. All rights reserved. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Brazil Division of Dermatology, Department of Internal Medicine, School of Medicine of Ribeirao Preto, University of Sao Paulo, Brazil State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, China Dana-Farber Cancer Institute, Harvard Medical School, Boston, USA Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Brazil Pulmonary and Critical Care Medicine Division, Department of Internal Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA Federal University of Triangulo Mineiro, Uberaba, Brazil. School of Pharmaceutical Sciences of Araraquara, Sao Paulo State University (UNESP), Brazil