Artigo
Expression studies in the embryo and in the micropylar endosperm of germinating coffee (Coffea arabica cv. Rubi) seeds
Registro en:
Plant Growth Regulation. Dordrecht: Springer, v. 75, n. 2, p. 575-581, 2015.
0167-6903
10.1007/s10725-014-9960-6
WOS:000349970000019
Autor
Farias, E. T. de [UNESP]
Amaral da Silva, E. A. [UNESP]
Toorop, Peter E.
Bewley, J. Derek
Hilhorst, Henk W. M.
Resumen
Germination of coffee (Coffea arabica L.) seed is slow and uneven. Its germination is the net result of events that occur simultaneously in the embryo and endosperm and which are controlled by abscisic acid (ABA). The aim of the study was to monitor the expression of genes related to the cell cycle and to cell wall modifications, including an actin (ACT), a cyclin-dependent kinase (CDK2a) and alpha-expansin (alpha-EXP) in the embryo, and alpha-galactosidase (alpha-GAL), beta-mannosidase (LeMSIDE2), endo-beta-mannanase (MANA) in the micropylar endosperm. The first seed germinated after 5 days of imbibition and 50 % germination was reached after 10 days. The embryo grew inside the seed prior to radicle protrusion and ABA inhibited both embryo growth and radicle protrusion. The expression of the genes associated with the embryo growth increased during germination and ABA partially inhibited expression. The expression of beta-mannosidase and endo-beta-mannanase increased during imbibition and ABA completely inhibited expression of these genes. However, alpha-galactosidase displayed a more constitutive expression and was less affected by ABA. ABA plays a dual role in the regulation of coffee seed germination; it concomitantly controls both endosperm weakening and embryo growth. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Universidade Estadual Paulista - UNESP,Departamento de Produção e Melhoramento Vegetal, Faculdade de Ciências Agronômicas, Botucatu, SP 18.610-307, Brazil Seed Conservation Department, Royal Botanic Gardens Kew, Wakehurst Place, Ardingly, West Sussex RH17 6TN, UK Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands Univ Estadual Paulista, Fac Ciencias Agron UNESP, Dept Prod &Melhoramento Vegetal, BR-18610307 Botucatu, SP, Brazil