Artigo
Impact of fipronil on the mushroom bodies of the stingless bee Scaptotrigona postica
Registro en:
Pest Management Science. Hoboken: Wiley-blackwell, v. 71, n. 1, p. 114-122, 2015.
1526-498X
10.1002/ps.3776
WOS:000346034600015
7538556085505819
0000-0002-1650-257X
Autor
Jacob, Cynthia R. O. [UNESP]
Soares, Hellen M. [UNESP]
Nocelli, Roberta C. F.
Malaspina, Osmar [UNESP]
Resumen
BACKGROUND: Studies on stingless bees are scarce, and little is known about these insects, especially regarding the effects of contamination by neurotoxic insecticides, which can cause damage to important structures of the insect brain. This study evaluated the morphological changes in the intrinsic neurons of the protocerebral mushroom bodies (Kenyon cells) of the stingless bee Scaptotrigona postica after exposure to different doses of fipronil, using light microscopy and transmission electron microscopy. This region of the brain was selected for analysis because of its importance as a sensory integration centre.RESULTS: In both oral and topical treatments, Kenyon cells presented pyknotic profiles, suggesting cell death. Statistical analysis showed significant differences among doses and exposure times. Transmission electron microscopy revealed changes in the nucleus and cellular organelles. Depending on the dose, the characteristics observed suggested apoptotosis or necrosis.CONCLUSION: This study demonstrates the toxic effects of fipronil. An increase in the number of pyknotic profiles of Kenyon cells of mushroom bodies was observed even at the sublethal doses of 0.27 ng AI bee(-1) and 0.24 ng AI mu L-1 in the topical and oral treatments respectively. Also, differences in the number of pyknotic profiles were dose and time dependent. (C) 2014 Society of Chemical Industry Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Univ Estadual Paulista Julio Mesquita Filho UNESP, Inst Biociencias, CEIS, Sao Paulo, SP, Brazil Univ Fed Sao Carlos, Ctr Ciencias Agr, Sao Paulo, SP, Brazil Univ Estadual Paulista Julio Mesquita Filho UNESP, Inst Biociencias, CEIS, Sao Paulo, SP, Brazil FAPESP: 10/03723-5