Anais e Proceedings de eventos
Analysis of fungal co-culture metabolites using both classic and imprinting Mass Spectrometry Imaging.
Registro en:
In: IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3., 2022, Rio de Janeiro. Book of abstracts. [S/l]: Sociedade Brasileira de Espectrometria de Massas, 2022.
Autor
TEIXEIRA, T. S.
RODRIGUES NETO, J. C.
CONCEIÇÃO, A. A.
COSTA, P. P. K. G.
SIQUEIRA, F. G. de
ABDELNUR, P. V.
Institución
Resumen
Fungi are able to produce a wide range of bioactive metabolites, which enhanced when challenged in co-culture[1]. For a better evaluation of these metabolites, the Mass Spectrometry Imaging (MSI) can be used to provide complementary information through the metabolite spatial localization [2]. However, some adaptations are required on available methodologies in MSI for applications in microrganisms[2], particularly on sample preparation. The imprinting method has been shown to be a robust method when applied to preparation of samples analyzed by DESI-MSI[3], but it has never been tested for MALDI-MSI for microrganisms, in our knowledge.