Artigo
Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis
Fecha
2010-12-01Registro en:
Aaps Journal. New York: Springer, v. 12, n. 4, p. 608-616, 2010.
1550-7416
10.1208/s12248-010-9224-y
WOS:000288426100014
Autor
Castro, Leandro Mantovani de [UNIFESP]
Berti, Denise A.
Russo, Lilian C.
Coelho, Veronica
Gozzo, Fabio C.
Oliveira, Vitor [UNIFESP]
Ferro, Emer Suavinho [UNIFESP]
Institución
Resumen
Cells produce and use peptides in distinctive ways. in the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role.