info:eu-repo/semantics/article
KH2PO4 improves cellulase production of Irpex lacteus and pycnoporus sanguineus
Fecha
2019-10-30Registro en:
Rodríguez, M.D., Alonso Paiva , I.M., Castrillo, M.L., Zapata, P.D. y Villalba, L.L. (2018). KH2PO4 improves cellulase production of Irpex lacteus and pycnoporus sanguineus. Journal of King Saud University-Science. Países Bajos : Elsevier Science, 31 (4), pp. 434-444.
1018-3647
N° 23611
A-CNyE-051
Autor
Rodríguez, María Daniela
Alonso Paiva, Iván Mathías
Castrillo, María Lorena
Zapata, Pedro Darío
Villalba, Laura Lidia
Institución
Resumen
The optimization of cellulase production by Irpex lacteus and Pycnoporus sanguineus was investigated. Fractional factorial design was conducted to determine significant variables and interactions. Response surface methodology was applied through Box-Behnken design to determine the optimum level of each factor on cellulase production. The optimal conditions of culture media were (g/L): for I. lacteus CaCl2.2(H2O) 0.3, MgSO4.7(H2O) 0.3 and KH2PO4 3, while for P. sanguineus was CaCl2.2(H2O) 0.1, MgSO4.7(H2O) 0.2 and KH2PO4 9. This optimized medium improved cellulase production, especially β-glucosidase activity values for I. lacteus. KH2PO4 was found in this work to be a useful mineral compound for cellulolytic production.