The number of acetylated proteins identified from bacteria to mammals has grown exponentially in the last ten years and it is now accepted that acetylation is a key component in most eukaryotic signaling pathways, as important as phosphorylation. The enzymes involved in this process are well described in mammals; acetyltransferases and deacetylases are found inside and outside the nuclear compartment and have different regulatory functions. In trypanosomatids several of these enzymes have been described and are postulated to be novel antiparasitic targets for the rational design of drugs. In this review article we present an update of the most important known acetylated proteins in trypanosomatids analyzing the acetylomes available. Also, we summarize the information available regarding acetyltransferases and deacetylases in trypanosomes and their potential use as chemotherapeutic targets."The published manuscript is available at EurekaSelect via https://www.eurekaselect.com/openurl/content.php?genre=article&doi=insert. DOI http://dx.doi.org/10.2174/0929867328666211126145721"Fil: Martinez Peralta, Gonzalo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.Fil: Serra, Esteban C. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.Fil: Alonso, Victoria Lucía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.Fil: Martinez Peralta, Gonzalo. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.Fil: Serra, Esteban C. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.Fil: Alonso, Victoria Lucía. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.