Fil: Lin, Yipu. The Francis Crick Institute, London; Reino Unido.Fil: Wharton, Stephen A. The Francis Crick Institute, London; Reino Unido.Fil: Whittaker, Lynne. The Francis Crick Institute, London; Reino Unido.Fil: Dai, Mian. The Francis Crick Institute, London; Reino Unido.Fil: Ermetal, Burcu. The Francis Crick Institute, London; Reino Unido.Fil: Lo, Janice. Centre for Health Protection, Department of Health, Hong Kong Special Administrative Region; China.Fil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Daniels, Rodney S. The Francis Crick Institute, London; Reino Unido.Fil: McCauley, John W. The Francis Crick Institute, London; Reino Unido.Two new subclades of influenza A(H3N2) viruses became prominent during the 2014-2015 Northern Hemisphere influenza season. The HA glycoproteins of these viruses showed sequence changes previously associated with alterations in receptor-binding properties. To address how these changes influence virus propagation, viruses were isolated and propagated in conventional MDCK cells and MDCK-SIAT1 cells, cells with enhanced expression of the human receptor for the virus, and analysed at each passage.