For macroinvertebrates inhabiting lentic systems, quantity and availability of various substrates are important for the establishment and development of different assemblages, also, the biome reflects macroinvertebrates system production. In this study, the diversity was estimated from the biomass present in the four substrates: macrophyte (Myriophyllum aquaticum), rock, sediments and organic matter, it was analyzed the seasonal variation of biomass and abundance considering three hydrological periods. It was collected 2.957 individuals grouped in 17 genera and 11 orders. Helobdella was the dominant taxon, it contributes to 90% of the total biomass of the community; Hyalella, Tanytarsus and Centrocorisa were important too. Electric conductivity, sulfates and the water temperature were determined for the biomass. The dominance of macroinvertebrates was higher in macrophyta (0,99). In the rest of the substrates, diversity was larger (ï = 1,5 nats.ind-1) and the evenness minor (ï = 0,24). There are significant differences in the registers of biomass among the substrates but not among the campaigns. In conclusion, macrophyte was the substrate and the habitat most used by macroinvertebrates and it contains more individuals; however, the macrophyte was not the most diversity substrates because, when it was compared with other three substrates the diversity is higher.mporal de la biomasa y la abundancia con base en tres períodos hidrológicos. Se colectaron 2.957 individuos (17 géneros, 11 órdenes). Helobdella fue el taxón dominante aportando 90 % a la biomasa total de la comunidad, seguido de Hyalella, Tanytarsus y Centrocorisa. La conductividad, los sulfatos y la temperatura hídrica fueron determinantes para la biomasa. En la macrófita se encontró menor diversidad de Hâ (0,03 nats.ind-1) y mayor dominancia (0,99); en el resto de sustratos la diversidad fue mayor (ï = 1,5) y la dominancia menor (ï = 0,24). Se encontraron diferencias significativas en los registros de biomasa entre sustratos más no entre campañas. En conclusión, el sustrato macrófita es el hábitat más utilizado por los macroinvertebrados pues alberga mayor cantidad de individuos; sin embargo, no presenta la mayor diversidad, a diferencia de los otros tres sustratos que evidencian mayor diversidad de taxones.