Assesment of DNA damage in two colombian populations of agricultirist and floriculturist
Evaluación del daño en el ADN en dos poblaciones colombianas de agricultores y floricultores
| dc.creator | Muñoz Aristizábal, Andrés Felipe | |
| dc.date | 2009-06-30 | |
| dc.date.accessioned | 2023-08-28T15:12:41Z | |
| dc.date.available | 2023-08-28T15:12:41Z | |
| dc.identifier | https://revistas.udca.edu.co/index.php/ruadc/article/view/637 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8442646 | |
| dc.description | Some research results prove the potential genotoxic activity of pesticides on human beings, which makes the development of methodologies that allow the determination of capacity of these substances to damage the DNA necessary. The comet assay is an economic, rapid and sensitive method that allows to detect, mainly, single strand breaks and alkaly-labil sites in individual cells. The aim of the present study was to evaluate the DNA damage by means of the comet assay in populations engaged in floriculture and agriculture activities, with variable exposure to pesticides, compared with a control group. Peripheral blood samples from a total of 101 individuals were obtained. The comet assay was performed using total peripheral blood as well as isolated lymphocytes. For this procedure the samples were embedded in agarose and set on a microscope slide, exposed to a lyses solution with detergent and finally to electrophoresis with alkaline buffer. Measuring the length of the comet tail (DNA migration) and the morphology of the comets, the DNA damage was estimated. In the exposed farmers, the comet assay revealed a statistically significant increase in DNA damage (P<0.001) versus floriculturists and the control group, but no difference between these later two groups were observed. The results suggest that the comet assay could be a reliable biomarker for occupational exposure to pesticides and that it could also be useful for similar studies. | en-US |
| dc.description | Algunas investigaciones demuestran el potencial genotóxico de ciertos plaguicidas sobre los seres humanos, lo cual, hace necesario el desarrollo de metodologías que permitan evaluar el impacto de dichas sustancias, para alterar el ADN. El ensayo del cometa alcalino es un método económico, rápido y sensible que detecta, principalmente, rompimientos de cadena sencilla y sitios lábiles al-álcali en células individuales. El objetivo del presente estudio fue evaluar el da?o en el ADN, en una población de floricultores y agricultores ocupacionalmente en contacto con plaguicidas y compararlo con un grupo control. Se obtuvieron muestras de sangre periférica de 101 personas, entre trabajadores expuestos e individuos del grupo control. El ensayo del cometa, se realizó utilizando sangre total y linfocitos aislados, con muestras embebidas en agarosa y puestas sobre una lámina portaobjetos; luego expuestas a una solución de lisis con detergente y, posteriormente, sometidas a una corriente eléctrica, con buffer alcalino. El da?o en el ADN fue evaluado por medición del largo de la cola (migración de ADN) y por la morfología que presentaban los cometas. En los agricultores, el ensayo mostró un incremento significativo (p < 0,001) del da?o en el ADN, comparado con los floricultores y el grupo control; entre estos últimos, no se hallaron diferencias. Los resultados sugieren que el ensayo de cometa puede ser un buen biomarcador de exposición ocupacional a plaguicidas y que puede ser utilizado para este tipo de estudios. | es-ES |
| dc.format | application/pdf | |
| dc.format | text/html | |
| dc.language | spa | |
| dc.publisher | Universidad de Ciencias Aplicadas y Ambientales U.D.C.A | es-ES |
| dc.relation | https://revistas.udca.edu.co/index.php/ruadc/article/view/637/621 | |
| dc.relation | https://revistas.udca.edu.co/index.php/ruadc/article/view/637/622 | |
| dc.relation | /*ref*/ÁLVAREZ-MOYA, C.; SANTERRE-LUCAS, A.; ZÚ?IGA-GONZÁLEZ, G.; TORRES-BUGARÍN, O.; PADILLA-CAMBEROS, E.; FERIA-VELASCO. A. 2001. Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia. Salud Publica Mex. 43:563-569. | |
| dc.relation | /*ref*/ANDERSON, D.; YU, T.W.; PHILLIPS, B.J.; SCHMEZER, P. 1994. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutat. Res. 307(1):261-271. | |
| dc.relation | /*ref*/ANTONUCCI, G.A.; COLUS, IM. 2000. Chromosomal aberrations analysis in a Brazilian population exposed to pesticides. Teratog. Carcinog. Mutag. 20(2):265-272. | |
| dc.relation | /*ref*/BLAIR, A.; CANTOR, K.P.; ZAHM, S.H. 1998. Non-Hodgkin’s lymphoma and agricultural use of the insecticide lindane. Am. J. Ind. Med. 33:82-87. | |
| dc.relation | /*ref*/BLASIAK, J.; JALOSZYNSKI, P.; TRZECIAK, A.; SZYFTER, K. 1999. In vitro studies on the genotoxicity of the organophosphorus insecticide malathion and its two analogues. Mutat. Res. 445(2):275-283. | |
| dc.relation | /*ref*/CARBONELL, E.; PUIG, M.; XAMENA, N.; CREUS, A.; MARCOS, R. 1990. Sister chromatid exchanges in lymphocytes of agricultural workers exposed to pesticides. Mutagenesis. 5(3):403-405. | |
| dc.relation | /*ref*/CLEMENTS, C.; RALPH, S.; PETRAS, M. 1997. Genotoxicity of selected herbicides in Rana catesbiana tadpoles using alkaline single-cell electrophoresis (comet) assay. Environ. Mol. Mutagen. 29(4):277-288. | |
| dc.relation | /*ref*/COMUNIDAD ANDINA CAN. Decisión 436.2002. Manual Técnico Andino para el registro y control de pesticidas químicos de uso agrícola. Lima, Perú: CAN, 296 de Junio de 2002. | |
| dc.relation | /*ref*/DAVIES, H.W.; KENNEDY, S.M.; TESCHKE, K.; QUINTANA, P.J. 1998. Cytogenetic analysis of South Asian berry pickers in British Columbia using the micronucleus assay in peripheral lymphocytes. Mutat. Res. 416(1):101-113. | |
| dc.relation | /*ref*/DE ROOS, A.J.; ZAHM, S.H.; CANTOR, K.P.; WEISENBURGER, D.D.; HOLMES, F.F.; BURMEISTER, L.F.; BLAIR, A. 2003. Integrative assessment of multiple pesticides as risk factors for non-Hodgkin’s lymphoma among men. Occup. Environ. Med. 60:e11. | |
| dc.relation | /*ref*/ELHAJOUJI, A. 2001. Use of the alkaline comet assay for industrial genotoxicity screening. Food Chemical Tox. 39(8):843-858. | |
| dc.relation | /*ref*/FAIRBAIRN, D.; OLIVE, P.L.; O’NEILL, K.L. 1995. The comet assay: a comprehensive review. Mutat. Res. 339(1):37-59. | |
| dc.relation | /*ref*/GARAJ-VRHOVAC, V.; ZELJEZIC, D. 1999. Chromosomal aberrations and frecuency of micronuclei in workers employed in pesticides production. Biologia. 54:707-712. | |
| dc.relation | /*ref*/GARAJ-VRHOVAC, V.; ZELJEZIC, D. 2000. Evaluation of DNA damage in workers occupationally exposed to pesticides using single-cell gel electrophoresis (SCGE) assay. Pesticide genotoxicity revealed by comet assay. Mutat. Res. 469(2):279-285. | |
| dc.relation | /*ref*/GARAJ-VRHOVAC, V.; ZELJEZIC, D. 2001. Cytogenetic monitoring of croatian population occupationally exposed to a complex mixture of pesticides. Toxicology. 165(2-3):153-162. | |
| dc.relation | /*ref*/GARAJ-VRHOVAC, V.; ZELJEZIC, D. 2002. Assessment of genome damage in a population of Croatian workers employed in pesticides production by chromosomal aberration analysis, micronucleus assay and comet assay. J. Appl. Tox. 22:248-255. | |
| dc.relation | /*ref*/GÓMEZ, S.; NORIEGA, N.; OSORIO, A.; GALICIA, F.; LING, S.; VILLALOBOS, R. 1992. Sister chromatid exchanges in a rural population of Mexico exposed to pesticides. Mutat. Res. 281:173-179. | |
| dc.relation | /*ref*/GÓMEZ-ARROYO, S.; DÍAZ-SANCHEZ, Y.; MENÉSES-PÉREZ, M.A.; VILLALOBOS-PIETRINI, R.; DELEÓN-RODRÍGUEZ, J.D. . Cytogenetic biomonitoring in a Mexican floriculture worker group exposed to pesticides. Mutat. Res. 466:117-124. | |
| dc.relation | /*ref*/GROVER, P.; DANADEVI, K.; MAHBOOB, M.; ROZATI, R.; BANU, S.; RAHMAN, M. 2003. Evaluation of genetic damage in workers employed in pesticide production utilizing the Comet assay. Mutagenesis. 18(2):201-205. | |
| dc.relation | /*ref*/IARC International Agency for Research on Cancer Monographs. 1991. Occupational Exposures in Insecticide Application and Some Pesticides. 53:45-93. | |
| dc.relation | /*ref*/KASSIE, F.; PARZEFALL, W.; KNASMULLER, S. 2000. Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat. Res. 463:13-31. | |
| dc.relation | /*ref*/LEBAILLY, P.; VIGREUX, C.; LECHEVREL, C.; LEDEMENEY, D.; GODARD, T.; SICHEL, F.; LETALAER, JY.; HENRY-AMAR, M.; GAUDUCHON, P. 1998. DNA damage in mononuclear leukocytes of farmers measured using the alkaline comet assay: modifications of DNA damage levels after a one-day field spraying period with selected pesticides. Cancer Epidemiol Biomarkers Prev. 7(10):929-940. | |
| dc.relation | /*ref*/LUCERO, L.; PASTOR, S.; SUÁREZ, S.; DURBAN, R.; GÓMEZ, C.; PARRON, T.; CREUS, A.; MARCOS, R. 2000. Cytogenetic biomonitoring of Spanish greenhouse workers exposed to pesticides: micronuclei analysis in peripheral blood lymphocytes and buccal epithelial cells. Mutat. Res. 464:255-262. | |
| dc.relation | /*ref*/MCKELVEY, M.; VALERIE, J. 1998. Emerging applications of the single cell gel electrophoresis (Comet) assay. Mutagenesis. 13(1):1-8. | |
| dc.relation | /*ref*/MERHI, M.; RAYNAL, H.; CAHUZAC, E.; VINSON, F.; CRAVEDI, J.P.; GAMET-PAYRASTRE, L. 2007. Occupational exposure to pesticides and risk of hematopoietic cancers: meta-analysis of case-control studies. Cancer Causes Control. 18(10):1209-1226. | |
| dc.relation | /*ref*/MESTER, B.; NIETERS, A.; DEEG, E.; ELSNER, G.; BECKER, N.; SEIDLER, A. 2006. Occupation and malignant lymphoma: a population based case control study in Germany. Occup. Environ. Med. 63:17-26. | |
| dc.relation | /*ref*/MINISTERIO DE SALUD. Decreto 1843 del 22 de julio de 1991. Disposiciones sanitarias sobre uso y manejo de plaguicidas. Bogotá: Ministerio de Salud; 1991. p.1-69. | |
| dc.relation | /*ref*/MOLLER, P.; KNUDSEN, L.E.; LOFT, S.; WALLIN, H. 2000. The comet assay as a rapid test in biomonitoring occupational exposure to DNA- agents and effect of confounding factors. Cancer Epidemiol. Biomarkers Prev. 9(10):1005-1015. | |
| dc.relation | /*ref*/MORETTI, M.; MARCARELLI, M.; VILLARINI, M.; FATIGONI, C.; SCASSELLATI-SFORZOLINI, G.; PASQUINI, R. 2002. In vitro testing for genotoxicity of the herbicide terbutryn: cytogenetic and primary DNA damage. Toxicol In Vitro. 16:81-88. | |
| dc.relation | /*ref*/OLIVE, P.L.; CHAN, A.P.; CU, C.S. 1988. Comparison between the DNA precipitation and alkali unwinding assays for detecting DNA strand breaks and cross-links. Cancer Res. 48:6444-6449. | |
| dc.relation | /*ref*/ORSI, L.; TROUSSARD, X.; MONNEREAU, A.; BERTHOU, C.; FENAUX, P.; MARIT, G.; SOUBEYRAN, P.; HUGUET, F.; MILPIED, N.; LEPORRIER, M.; HEMON, D.; CLAVEL, J. 2007. Occupation and lymphoid malignancies: results from a French case-control study. J. Occup. Environ. Med. 49(12):1339-50. | |
| dc.relation | /*ref*/OSTLING, O.; JOHANSON, K.L. 1984. Microelectrophoretic study of radiation-induced DNA damages in individual mamalian cells. Biochem. Biophys. Res. Commun. 123:291-298. | |
| dc.relation | /*ref*/PASTOR, S.; GUTIÉRREZ, S.; CREUS, A.; CEBULSKA-WASILEWSKA, A.; MARCOS, R. 2001a. Micronuclei in peripheral blood lymphocytes and buccal epithelial cells of Polish farmers exposed to pesticides. Mutat. Res. 495:147-156. | |
| dc.relation | /*ref*/PASTOR, S.; GUTIÉRREZ, S.; CREUS, A.; XAMENA, N.; PIPERAKIS, S.; MARCOS, R. 2001b. Cytogenetic analysis of Greek farmers using the micronucleus assay in peripheral lymphocytes and buccal cells. Mutagenesis. 16:539-545. | |
| dc.relation | /*ref*/RAHMAN, M.F.; MAHBOOB, M.; DANADEVI, K.; BANU, B.S.; GROVER, P. 2002. Assessment of genotoxic effects of chloropyriphos and acephate by the comet assay in mice leucocytes. Mutat. Res. 516(1):139-147. | |
| dc.relation | /*ref*/ROJAS, E.; LÓPEZ, M.C.; VALVERDE, M. 1999. Single cell gel electrophoresis assay: methodology and applications. J. Chromatography. 722:225-254. | |
| dc.relation | /*ref*/SHAH, R.G.; LAGUEUX, J.; KAPUR, S.; LEVALLOIS, P.; AYOTTE, P.; TREMBLAY, M. 1997. Determination of genotoxicity of the metabolites of pesticides guthion, sencor, lorox, reglone, daconil and admire by P32-postlabeling. Mol. Cell. Biochem. 169:177-184. | |
| dc.relation | /*ref*/SINGH, N.P.; COY, M.I.; TICE, R.; SCHNEIDER, E.L. 1988. A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 175:184-191. | |
| dc.relation | /*ref*/TICE, R.R. 2000. Single Cell Gel/Comet Assay: Guidelines for In Vitro and In Vivo aspects of Genetic Toxicology. Environ. Mol. Mutagen. 35:206-221. | |
| dc.relation | /*ref*/TISCH, M.; SCHMEZER, P.; FAULDE, M.; GROH, A.; MAIER, H. 2002. Genotoxicity studies on permethrin, DEET and diazinon in primary human nasal mucosal cells. Eur. Arch. Otorhinolaryngol. 259(3):150-153. | |
| dc.relation | /*ref*/VALVERDE, M.; LÓPEZ, A.D.L.; LÓPEZ, I.; SÁNCHEZ, I.; FORTOUL, T.I.; OSTROSKY-REPETTO, R.; BALIGA, S.S. 1997. DNA damage in leukocytes and buccal and nasal epithelial cells of individuals exposed to air pollution in Mexico City. Environ. Mol. Mutagen. 30(2):147-152. | |
| dc.relation | /*ref*/VARONA, M.; CÁRDENAS, O.; CRANE, C.; ROCHA, S.; CUERVO, G.; VARGAS, J. 2003. Cytogenetic alterations in field workers routinely exposed to pesticides in Bogotá flowers farms. Biomédica. 23(2):141-152. | |
| dc.relation | /*ref*/VARONA, M.; TOLOSA, J.; CÁRDENAS, O.; TORRES, C.; PARDI, D.; CARRASQUILLA, G.; FRUMKIN, H. 2005. Descripción del uso y manejo de plaguicidas en las empresas de flores afiliadas a Asocolflores. Biomédica. 25:377-389 | |
| dc.relation | /*ref*/VIEL, J.F.; CHALLIER, B.; PITARD, A.; POBEL, D. 1998. Brain cancer mortality among French farmers: the vineyard pesticide hypothesis. Arch. Environ. Health. 53:65-70. | |
| dc.relation | /*ref*/VILLARINI, M.; SCASSELLATI-SFORZOLINI, G.; MORETTI, M.; PASQUINI, R. 2002. In vitro genotoxicity of terbutryn evaluated by the alkaline single-cell microgel-electrophoresis "comet"; assay. Cell Biol. Toxicol. 16(5):285-292. | |
| dc.relation | /*ref*/WEISENBURGER, D.S.; HARRINGTON, J.O. 1990. Armitage, B-cell neoplasia: a conceptual understanding based on the normal humoral immune response. Pathol. Annu. 25:99-115. | |
| dc.relation | /*ref*/ZELJEZIC, D.; GARAJ-VRHOVAC, V. 2001. Chromosomal aberration and single cell gel electrophoresis (Comet) assay in the longitudinal risk assessment of occupational exposure to pesticides. Mutagenesis. 16(4):359-363. | |
| dc.source | Revista U.D.C.A Actualidad & Divulgación Científica; Vol. 12 No. 1 (2009): Revista U.D.C.A Actualidad & Divulgación Científica. Enero-Junio; 7-16 | en-US |
| dc.source | Revista U.D.C.A Actualidad & Divulgación Científica; Vol. 12 Núm. 1 (2009): Revista U.D.C.A Actualidad & Divulgación Científica. Enero-Junio; 7-16 | es-ES |
| dc.source | Revista U.D.C.A Actualidad & Divulgación Científica; v. 12 n. 1 (2009): Revista U.D.C.A Actualidad & Divulgación Científica. Enero-Junio; 7-16 | pt-BR |
| dc.source | 2619-2551 | |
| dc.source | 0123-4226 | |
| dc.source | 10.31910/rudca.v12.n1.2009 | |
| dc.subject | Genotoxicidad | es-ES |
| dc.subject | Ensayo del Cometa | es-ES |
| dc.subject | Plaguicidas | es-ES |
| dc.subject | Exposición ocupacional | es-ES |
| dc.subject | Genotoxicity | en-US |
| dc.subject | Comet assay | en-US |
| dc.subject | Pesticides | en-US |
| dc.subject | Occupational exposure | en-US |
| dc.title | Assesment of DNA damage in two colombian populations of agricultirist and floriculturist | en-US |
| dc.title | Evaluación del daño en el ADN en dos poblaciones colombianas de agricultores y floricultores | es-ES |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion |