Three-dimensional assessment of enamel and dentine in mouse molar teeth during masseter muscle hypofunction

Revista de Estomatología

dc.creatorBalanta-Melo, Julián
dc.creatorBemmann, Maximilian
dc.creatorToro-Ibacache, Viviana
dc.creatorKupczik, Kornelius
dc.creatorBuvinic-Radic, Sonja Milena
dc.date2021-08-23T22:56:28Z
dc.date2022-07-07T02:36:39Z
dc.date2021-08-23T22:56:28Z
dc.date2022-07-07T02:36:39Z
dc.date2018
dc.date.accessioned2023-08-22T23:48:15Z
dc.date.available2023-08-22T23:48:15Z
dc.identifier1151353
dc.identifier1151353
dc.identifierhttps://hdl.handle.net/10533/251862
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8352617
dc.descriptionBackground: Mouse molar is a widely used model for teeth development. However, the effect of masticatory function on enamel and dentine in adult individuals remains poorly understood. As reported, the unilateral masseter hypofunction induced by botulinum toxin type A (BoNTA) resulted in mandibular bone damage and signs of unilateral chewing in adult mice. Objective: We aimed to assess the amount of enamel and dentine in the first molar (M1) during the unilateral masseter hypofunction in mice, using high-resolution X-ray microtomography (µCT) as threedimensional approach. Materials and methods: Mandibles of adult BALB/c mice, located either in a Control-group (without intervention) or a BoNTA-group, were ex-vivo scanned using µCT. Treated individuals received each one BoNTA intervention in the right masseter, and saline solution in the left masseter (intra-individual control). Enamel and dentine from M1 were segmented, and volume, thickness and mesial root length were quantified. Results: Enamel volume from treated side resulted unchanged after 2 weeks of unilateral masseter hypofunction. No differences for enamel volume were found between both sides of control individuals, and between these and samples from hypofunctional side in BoNTA-group. Enamel volume from saline-injected side was reduced when compared with experimental side (p<0,01). No differences in dentine volume, thickness of enamel and dentine, and mesial root length were found for any group. Conclusion: The amount of enamel in hypofunctional molars remains unaffected after unilateral BoNTA intervention in the masseter, but contralateral side showed reduced enamel volume. Therefore, increased functional wearing during unilateral chewing after BoNTA intervention should be considered. Key words: Mastication, botulinum toxin type A, X-ray microtomography, unilateral chewing
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.25100/re.v26i2.7634
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThree-dimensional assessment of enamel and dentine in mouse molar teeth during masseter muscle hypofunction
dc.titleRevista de Estomatología
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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