The activation of metabotropic glutamate 5 receptors in the rat ventral tegmental area increases dopamine extracellular levels

Neuroreport

dc.creatorFerrada, Carla
dc.creatorSotomayor-Zárate, Ramón
dc.creatorAbarca, Jorge
dc.creatorGysling-Caselli, Katia Cecilia
dc.date2021-08-23T22:58:55Z
dc.date2022-07-07T14:55:46Z
dc.date2021-08-23T22:58:55Z
dc.date2022-07-07T14:55:46Z
dc.date2017
dc.date.accessioned2023-08-22T22:46:48Z
dc.date.available2023-08-22T22:46:48Z
dc.identifier1150244
dc.identifier1150244
dc.identifierhttps://hdl.handle.net/10533/252449
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8348388
dc.descriptionThe mesocorticolimbic circuit projects to the prefrontal cortex, hippocampus, amygdala, and nucleus accumbens, among others, and it originates in the dopaminergic neurons of the ventral tegmental area (VTA). The VTA receives glutamatergic inputs from the prefrontal cortex and several subcortical regions. The glutamate released activates dopaminergic neurons and its action depends on the activation of ionotropic and metabotropic glutamate receptors. VTA dopaminergic neurons release dopamine (DA) from axon terminals in the innervated regions and somatodendritically in the VTA itself. DA release in the VTA is directly correlated with the activity of dopaminergic neurons. We hypothesized that metabotropic glutamate 5 receptors (mGlu(5)) directly regulate the activity of VTA dopaminergic neurons. To test this hypothesis, the extracellular levels of VTA DA and glutamate were studied by in-vivo microdialysis after an intra-VTA perfusion of (R,S)-2-chloro-5-hydroxyphenylglycine (CHPG), selective mGlu(5) agonist. We observed that CHPG induced a significant increase in VTA DA and glutamate extracellular levels. To determine whether the effect of CHPG on DA levels is because of the increase in glutamate release, we perfused kynurenic acid, an ionotropic glutamate receptor antagonist, through the probe. Our results showed that kynurenic acid did not block the ability of CHPG to cause DA release. Thus, our results suggest that CHPG acts directly on mGlu(5) in dopaminergic neurons to induce the release of DA. Copyright (C) 2016 Wolters Kluwer Health, Inc. All rights reserved. Keywords. Author Keywords:metabotropic glutamate receptor; mGlu(5); (R,S)-2-chloro-5-hydroxyphenylglycine; ventral tegmental area . KeyWords Plus:NUCLEUS-ACCUMBENS SHELL; LONG-TERM POTENTIATION; SYNAPTIC PLASTICITY; POSTSYNAPTIC DENSITY; PREFRONTAL CORTEX; LATERAL SEPTUM; MGLUR5; COCAINE; RELEASE; NMDA
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.1097/WNR.0000000000000708
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThe activation of metabotropic glutamate 5 receptors in the rat ventral tegmental area increases dopamine extracellular levels
dc.titleNeuroreport
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución