Serendipita indica E5 ' NT modulates extracellular nucleotide levels in the plant apoplast and affects fungal colonization

EMBO Reports

dc.creatorNizam, Shadab
dc.creatorQiang, Xiaoyu
dc.creatorWawra, Stephan
dc.creatorNostadt, Robin
dc.creatorGetzke, Felix
dc.creatorSchwanke, Florian
dc.creatorDreyer, Ingo
dc.creatorLangen, Gregor
dc.creatorZuccaro, Alga
dc.date2021-08-23T22:49:47Z
dc.date2022-07-08T20:23:31Z
dc.date2021-08-23T22:49:47Z
dc.date2022-07-08T20:23:31Z
dc.date2019
dc.date.accessioned2023-08-22T11:50:10Z
dc.date.available2023-08-22T11:50:10Z
dc.identifier1150054
dc.identifier1150054
dc.identifierhttps://hdl.handle.net/10533/250460
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8345708
dc.descriptionExtracellular adenosine 5'-triphosphate (eATP) is an essential signaling molecule that mediates different cellular processes through its interaction with membrane-associated receptor proteins in animals and plants. eATP regulates plant growth, development, and responses to biotic and abiotic stresses. Its accumulation in the apoplast induces ROS production and cytoplasmic calcium increase mediating a defense response to invading microbes. We show here that perception of extracellular nucleotides, such as eATP, is important in plant-fungus interactions and that during colonization by the beneficial root endophyte Serendipita indica eATP accumulates in the apoplast at early symbiotic stages. Using liquid chromatography-tandem mass spectrometry, and cytological and functional analysis, we show that S. indica secrets SiE5'NT, an enzymatically active ecto-5'-nucleotidase capable of hydrolyzing nucleotides in the apoplast. Arabidopsis thaliana lines producing extracellular SiE5'NT are significantly better colonized, have reduced eATP levels, and altered responses to biotic stresses, indicating that SiE5'NT functions as a compatibility factor. Our data suggest that extracellular bioactive nucleotides and their perception play an important role in fungus-root interactions and that fungal-derived enzymes can modify apoplastic metabolites to promote fungal accommodation. Keywords. Author Keywords:DORN1
dc.descriptionextracellular nucleotides
dc.descriptionlectin receptor kinase LecRK-I.9
dc.descriptionPiriformospora indica
dc.descriptionpurinergic signaling
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttp://www.fch.unicen.edu.ar/ojs-3.1.0/index.php/estudios-socioterritoriales/article/download/302/271
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleSerendipita indica E5 ' NT modulates extracellular nucleotide levels in the plant apoplast and affects fungal colonization
dc.titleEMBO Reports
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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