Ultrastructural Variability of the Exosporium Layer of Clostridium difficile Spores

APPLIED AND ENVIRONMENTAL MICROBIOLOGY

dc.creatorPizarro-Guajardo, Marjorie
dc.creatorCalderón-Romero, Paulina F
dc.creatorCastro-Cordova, Pablo
dc.creatorMora-Uribe, Paola
dc.creatorParedes-Sabja, Daniel Gonzalo
dc.date2021-08-23T22:53:44Z
dc.date2022-07-08T20:40:16Z
dc.date2021-08-23T22:53:44Z
dc.date2022-07-08T20:40:16Z
dc.date2016
dc.date.accessioned2023-08-22T11:41:47Z
dc.date.available2023-08-22T11:41:47Z
dc.identifier1151025
dc.identifier1151025
dc.identifierhttps://hdl.handle.net/10533/251235
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8345134
dc.descriptionThe anaerobic sporeformer Clostridium difficile is the leading cause of nosocomial antibiotic-associated diarrhea in developed and developing countries. The metabolically dormant spore form is considered the transmission, infectious, and persistent morphotype, and the outermost exosporium layer is likely to play a major role in spore-host interactions during the first contact of C.difficile spores with the host and for spore persistence during recurrent episodes of infection. Although some studies on the biology of the exosporium have been conducted (J. Barra-Carrasco et al., J Bacteriol 195: 3863-3875, 2013, http://dx.doi.org/10.1128/JB.00369-13; J. Phetcharaburanin et al., Mol Microbiol 92: 1025-1038, 2014, http://dx.doi.org/10.1111/mmi.12611), there is a lack of information on the ultrastructural variability and stability of this layer. In this work, using transmission electron micrographs, we analyzed the variability of the spore's outermost layers in various strains and found distinctive variability in the ultrastructural morphotype of the exosporium within and between strains. Through transmission electron micrographs, we observed that although this layer was stable during spore purification, it was partially lost after 6 months of storage at room temperature. These observations were confirmed by indirect immunofluorescence microscopy, where a significant decrease in the levels of two exosporium markers, the N-terminal domain of BclA1 and CdeC, was observed. It is also noteworthy that the presence of the exosporium marker CdeC on spores obtained from C. difficile biofilms depended on the biofilm culture conditions and the strain used. Collectively, these results provide information on the heterogeneity and stability of the exosporium surface of C. difficile spores. These findings have direct implications and should be considered in the development of novel methods to diagnose and/or remove C. difficile spores by using exosporium proteins as targets.
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.1128/AEM.03410-15
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleUltrastructural Variability of the Exosporium Layer of Clostridium difficile Spores
dc.titleAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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