Potassium current in mature bovine spermatozoa

SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE;
Syst. Biol. Reprod. Med.

dc.creatorSánchez-Gutierrez, Raul
dc.creatorRomero-Mejia, Fernando
dc.creatorMarconi-Toro, Marcelo
dc.creatorUlrich, Henning
dc.date2017-04-27T18:52:55Z
dc.date2022-07-07T02:25:08Z
dc.date2017-04-27T18:52:55Z
dc.date2022-07-07T02:25:08Z
dc.date2008
dc.date.accessioned2023-08-22T08:36:54Z
dc.date.available2023-08-22T08:36:54Z
dc.identifier0
dc.identifierD05I10416
dc.identifierD05I10416
dc.identifierWOS:000261382200002
dc.identifier1939-6368
dc.identifierhttps://hdl.handle.net/10533/197783
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8334314
dc.descriptionIon channels have been assigned a pivotal importance in various sperm functions and are therefore promising targets for contraceptive development. The lack of data on channel functionality and pharmacology has hampered this goal. This is a consequence of technical problems of applying electrophysiological techniques to spermatozoa due to their small size and form. By using a laminin coating to increase adherence of spermatozoa and nystatin in the patch pipette for pore formation, we have adapted the whole-cell recording technique to study currents in mature uncapacitated bovine spermatozoa. Employing these conditions, in the head region, patched spermatozoa could be transferred into the whole-cell configuration. For the first time we document an outward rectifying current in mature bovine spermatozoa was blocked by tetraethyl ammonium (TEA) chloride. The observation of a shift in the reversal potential as a response to changes in the extracellular concentration of K+ ions allowed us to identify this current as K+ selective. This result shows that K+ channels in the head region of mature uncapacitated bovine spermatozoa can be suitably investigated using the whole-cell recording patch-clamp technique.
dc.descriptionThis work was supported by Fondef - Conicyt ( No D05I10416) projects ( Chile). HU acknowledges funding from FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, project No. 2006/61285-9) and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Brazil. The authors wish to acknowledge Dr. Paulo Boschcov for his help in preparing the manuscript and Dr. Armanda Gameiro, Department of Chemistry, Binghampton University, NY, for comments on the manuscript.
dc.description5
dc.descriptionFONDEF
dc.descriptionhenning@iq.usp.br; fromero@ufro.cl
dc.descriptionFondef - Conicyt [D05I10416]; FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2006/61285-9]; CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Brazil
dc.description6
dc.descriptionFONDEF
dc.description54
dc.languageENG
dc.publisherINFORMA HEALTHCARE
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinfo:eu-repo/grantAgreement/Fondef/D05I10416
dc.relationinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relationhttps://doi.org/10.1080/19396360802419366
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titlePotassium current in mature bovine spermatozoa
dc.titleSYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
dc.titleSyst. Biol. Reprod. Med.
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.coverageLONDON


Este ítem pertenece a la siguiente institución