Identification and characterization of potentially algal-lytic marine bacteria strongly associated with the toxic dinoflagellate alexandrium catenella

JOURNAL OF EUKARYOTIC MICROBIOLOGY;
J. Eukaryot. Microbiol.

dc.creatorSUAREZ-ISLA, BENJAMON ALVARO
dc.creatorAMARO-SEPÚLVEDA, ANA
dc.creatorOGALDE, SANDRA
dc.creatorVENEGAS, JUAN
dc.date2017-04-27T18:49:15Z
dc.date2022-07-07T01:38:14Z
dc.date2017-04-27T18:49:15Z
dc.date2022-07-07T01:38:14Z
dc.date2005
dc.date.accessioned2023-08-22T05:03:38Z
dc.date.available2023-08-22T05:03:38Z
dc.identifier0
dc.identifierAQ02I1007
dc.identifierAQ02I1007
dc.identifierWOS:000229839300004
dc.identifier1066-5234
dc.identifierhttps://hdl.handle.net/10533/196817
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8322549
dc.descriptionThe toxic dinoflagellate Alexandrium catenella isolated from fjords in Southern Chile produces several analogues of saxitoxin and has been associated with outbreaks of paralytic shellfish poisoning. Three bacterial strains, which remained in close association with this dinoflagellate in culture, were isolated by inoculating the dinoflagellate onto marine agar. The phenotypically different cultivable bacterial colonies were purified. Their genetic identification was done by polymerase chain reaction amplification of the 16S rRNA genes. Partial sequence analysis suggested that the most probable affiliations were to two bacterial phyla: Proteobacteria and the Cytophaga group. The molecular identification was complemented by morphological data and biochemical profiling. The three bacterial species, when grown separately from phytoplankton cells in high-nutrient media, released algal-lytic compounds together with aminopeptidase, lipase, glucosaminidase, and alkaline phosphatase. When the same bacteria, free of organic nutrients, were added back to the algal culture they displayed no detrimental effects on the dinoflagellate cells and recovered their symbiotic characteristics. This observation is consistent with phylogenetic analysis that reveals that these bacteria correspond to species distinct from other bacterial strains previously classified as algicidal bacteria. Thus, bacterial-derived lytic activities are expressed only in the presence of high-nutrient culture media and it is likely that in situ environmental conditions may modulate their expression.
dc.description0
dc.description48
dc.descriptionFONDEF
dc.descriptionaamaro@med.uchile.cl
dc.description0
dc.description3
dc.descriptionFONDEF
dc.description52
dc.languageENG
dc.publisherBLACKWELL PUBLISHING INC
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinfo:eu-repo/grantAgreement/Fondef/AQ02I1007
dc.relationinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relationhttps://doi.org/10.1111/j.1550-7408.2005.00031.x
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleIdentification and characterization of potentially algal-lytic marine bacteria strongly associated with the toxic dinoflagellate alexandrium catenella
dc.titleJOURNAL OF EUKARYOTIC MICROBIOLOGY
dc.titleJ. Eukaryot. Microbiol.
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.coverageMALDEN


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