Subtyping of Clostridium difficile PCR ribotypes 591, 106 and 002, the dominant strain types circulating in Medellin, Colombia
PLOS ONE
| dc.creator | Salazar, Clara Lina | |
| dc.creator | Reyes, Catalina | |
| dc.creator | Cienfuegos-Gallet, Astrid Vanessa | |
| dc.creator | Best, Emma | |
| dc.creator | Atehortua, Santiago | |
| dc.creator | Sierra, Patricia | |
| dc.creator | Correa, Margarita M | |
| dc.creator | Fawley, Warren N | |
| dc.creator | Paredes-Sabja, Daniel Gonzalo | |
| dc.creator | Wilcox, Mark | |
| dc.creator | González, Ángel | |
| dc.date | 2021-08-23T22:53:37Z | |
| dc.date | 2022-07-08T20:39:54Z | |
| dc.date | 2021-08-23T22:53:37Z | |
| dc.date | 2022-07-08T20:39:54Z | |
| dc.date | 2018 | |
| dc.date.accessioned | 2023-08-22T04:54:20Z | |
| dc.date.available | 2023-08-22T04:54:20Z | |
| dc.identifier | 1151025 | |
| dc.identifier | 1151025 | |
| dc.identifier | https://hdl.handle.net/10533/251220 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8321847 | |
| dc.description | We aimed to achieve a higher typing resolution within the three dominant Clostridium difficile ribotypes (591,106 and 002) circulating in Colombia. A total of 50 C. difficile isolates we had previously typed by PCR-ribotyping, representing the major three ribotypes circulating in Colombia, were analyzed. Twenty-seven isolates of ribotype 591, 12 of ribotype 106 and 11 of ribotype 002 were subtyped by multiple locus variable-number tandem-repeat analysis (MLVA). The presence of the PaLoc genes (tcdA/tccB), toxin production in culture and antimicrobial susceptibility were also determined. From the total C. difficile ribotypes analyzed, 20 isolates (74%) of ribotype 591, nine (75%) of ribotype 106 and five (45.5%) of ribotype 002 were recovered from patients with Clostridium difficile infection (CDI). MLVA allowed us to recognize four and two different clonal complexes for ribotypes 591 and 002, respectively, having a summed tandem-repeat difference (STRD) <2, whereas none of the ribotype 106 isolates were grouped in a cluster or clonal complex having a STRD >10. Six ribotype 591 and three ribotype 002 isolates belonging to a defined clonal complex were isolated on the same week in two different hospitals. All ribotypes harbored either tcdA(+)/tccB(+) or tcdA(-)/tccB(+) PaLoc genes. Moreover, 94% of the isolates were positive for toxin in culture. All isolates were susceptible to vancomycin and metronidazole, while 75% to 100% of the isolates were resistant to clindamycin, and less than 14.8% of ribotype 591 isolates were resistant to moxifloxacina. No significant differences were found among ribotypes with respect to demographic and clinical patients' data; however, our results demonstrated a high molecular heterogeneity of C. difficile strains circulating in Colombia. | |
| dc.description | Regular 2015 | |
| dc.description | FONDECYT | |
| dc.description | FONDECYT | |
| dc.language | eng | |
| dc.relation | handle/10533/111557 | |
| dc.relation | handle/10533/111541 | |
| dc.relation | handle/10533/108045 | |
| dc.relation | https://doi.org/10.1371/journal.pone.0195694 | |
| dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
| dc.rights | info:eu-repo/semantics/article | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.title | Subtyping of Clostridium difficile PCR ribotypes 591, 106 and 002, the dominant strain types circulating in Medellin, Colombia | |
| dc.title | PLOS ONE | |
| dc.type | Articulo | |
| dc.type | info:eu-repo/semantics/publishedVersion |