Heterologous expression of a novel psychrophilic cu/zn superoxide dismutase from deschampsia antarctica

PROCESS BIOCHEMISTRY;
Process Biochem.

dc.creatorGUTIERREZ-MORAGA, ANA
dc.creatorGIDEKEL-BLUFSTEIN, MANUEL
dc.creatorBARBA-DE LA ROSA, ANA
dc.creatorDELEON-RODRIGUEZ, ANTONIO
dc.creatorGARCIA-ECHAURI, SERGIO
dc.creatorORDOÑEZ, LEANDRO
dc.creatorROJAS-CONTRERAS, JUAN
dc.date2017-04-27T18:52:41Z
dc.date2022-07-07T02:24:35Z
dc.date2017-04-27T18:52:41Z
dc.date2022-07-07T02:24:35Z
dc.date2009
dc.date.accessioned2023-08-22T04:38:49Z
dc.date.available2023-08-22T04:38:49Z
dc.identifier0
dc.identifierD03I1079
dc.identifierD03I1079
dc.identifierWOS:000268918300004
dc.identifier1359-5113
dc.identifierhttps://hdl.handle.net/10533/197736
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8320612
dc.descriptionSuperoxide dismutase (SOD) catalyzes the conversion of the superoxide radical ((center dot)O(2)(-)) into oxygen and hydrogen peroxide. Deschampsia antarctica is a plant that grows in Antarctica and survives to extreme low temperature and high UV radiation, thus it is an ideal model to study novel antioxidants. A cDNA Cu/Zn-SOD gene from D. antarctica was cloned into a pET vector and expressed in Escherichia coli BL21-SI. 112 mg/L of recombinant Cu/Zn-SOD was attained in batch cultures in bioreactor. Using Ni-affinity gel chromatography, the recombinant Cu/Zn-SOD was recovered with a purity of 90% and a specific enzyme activity of 749 at 25 degrees C. However, zymogram test showed that the enzyme has more activity at 4 degrees C. This D. antarctica SOD could be used to reduce the oxidation of refrigerated and frozen foods. (C) 2009 Elsevier Ltd. All rights reserved.
dc.descriptionThis work was partially financed by the grant Antarctica Des S-3607, DIUFRO D109-0081, D108-2002, FONDEF Grant D031-1079 and CONACyT Grant 82010. A. De Leon Rodriguez thanks to CONACyT for the sabbatical fellowship 79973 and Sergio A. Garcia thanks to CONACyT for his scholarship 209658. We thank to the Chilean Antarctic Institute (INACH) for the logistic support during the stay in the Scientific Base "Prof. Julio Escudero", King George Island, Antarctic.
dc.description5
dc.descriptionFONDEF
dc.descriptionaleonr@ipicyt.edu.mx
dc.descriptionAntarctica Des [S-3607]; DIUFRO [D109-0081, D108-2002]; FONDEF [D031-1079]; CONACyT [82010, 79973, 209658]
dc.description9
dc.descriptionFONDEF
dc.description44
dc.languageENG
dc.publisherELSEVIER SCI LTD
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinfo:eu-repo/grantAgreement/Fondef/D03I1079
dc.relationinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relationhttps://doi.org/10.1016/j.procbio.2009.04.021
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleHeterologous expression of a novel psychrophilic cu/zn superoxide dismutase from deschampsia antarctica
dc.titlePROCESS BIOCHEMISTRY
dc.titleProcess Biochem.
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.coverageOXFORD


Este ítem pertenece a la siguiente institución