ATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X(7) receptors

SCIENCE SIGNALING

dc.creatorSáez-Carreño, Juan Carlos
dc.creatorVargas, Pablo
dc.creatorShoji, Kenji F
dc.creatorHarcha, Paloma A
dc.creatorLennon-Dumenil, Ana María
dc.date2021-08-23T22:59:15Z
dc.date2022-07-07T14:56:15Z
dc.date2021-08-23T22:59:15Z
dc.date2022-07-07T14:56:15Z
dc.date2017
dc.date.accessioned2023-08-22T03:12:38Z
dc.date.available2023-08-22T03:12:38Z
dc.identifier1150291
dc.identifier1150291
dc.identifierhttps://hdl.handle.net/10533/252549
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8313893
dc.descriptionUpon its release frominjured cells, such as infected, transformed, inflamed, or necrotic cells, extracellular adenosine-5'-triphosphate (ATP) acts as a danger signal that recruits phagocytes, such as neutrophils, macrophages, and dendritic cells (DCs), to the site of injury. The sensing of extracellular ATP occurs through purinergic (P2) receptors. We investigated the cellular mechanisms linking purinergic signaling to DC motility. We found that ATP stimulated fast DC motility through an autocrine signaling loop, which was initiated by the activation of P2X(7) receptors and further amplified by pannexin 1 (Panx1) channels. Upon stimulation of the P2X(7) receptor by ATP, Panx1 contributed to fast DC motility by increasing the permeability of the plasma membrane, which resulted in supplementary ATP release. In the absence of Panx1, DCs failed to increase their speed of migration in response toATP, despite exhibiting a normal P2X(7) receptor-mediated Ca2+ response. In addition to DC migration, Panx1 channel-and P2X(7) receptor-dependent signalingwas further required to stimulate the reorganization of the actin cytoskeleton. In vivo, functional Panx1 channels were required for the homing of DCs to lymph nodes, although they were dispensable for DC maturation. These data suggest that P2X(7) receptors and Panx1 channels are crucial players in the regulation of DC migration to endogenous danger signals.
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.1126/scisignal.aah7107
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X(7) receptors
dc.titleSCIENCE SIGNALING
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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