Dynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray

MICROBIAL BIOTECHNOLOGY (ONLINE);
MICROB BIOTECHNOL

dc.creatorDemergasso-Semenzato, Cecilia
dc.creatorAcosta-Grinok, Mauricio
dc.creatorGalleguillos-Guajardo, Felipe
dc.creatorMoreno-Paz, M.,
dc.creatorParro, Víctor
dc.creatorRemonsellez-Fuentes, Francisco
dc.date2017-04-27T18:54:03Z
dc.date2022-07-07T02:28:16Z
dc.date2017-04-27T18:54:03Z
dc.date2022-07-07T02:28:16Z
dc.date2009
dc.date.accessioned2023-08-22T01:50:42Z
dc.date.available2023-08-22T01:50:42Z
dc.identifier0
dc.identifierD04I1169
dc.identifierD04I1169
dc.identifierWOS:000207904200005
dc.identifier1751-7915
dc.identifierhttps://hdl.handle.net/10533/198036
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8307916
dc.descriptionThe bioleaching of metal sulfide has developed into a very important industrial process and understanding the microbial dynamic is key to advancing commercial bioleaching operations. Here we report the first quantitative description of the dynamic of active communities in an industrial bioleaching heap. Acidithiobacillus ferrooxidans was the most abundant during the first part of the leaching cycle, while the abundance of Leptospirillum ferriphilum and Ferroplasma acidiphilum increased with age of the heap. Acidithiobacillus thiooxidans kept constant throughout the leaching cycle, and Firmicutes group showed a low and a patchy distribution in the heap. The Acidiphilium-like bacteria reached their highest abundance corresponding to the amount of autotrophs. The active microorganisms in the leaching system were determined using two RNA-based sensitive techniques. In most cases, the 16S rRNA copy numbers of At. ferrooxidans, L. ferriphilum, At. thiooxidans and F. acidiphilum, was concomitant with the DNA copy numbers, whereas Acidiphilium-like bacteria and some Firmicutes members did not show a clear correlation between 16S rRNA accumulation and DNA copy numbers. However, the prokaryotic acidophile microarray (PAM) analysis showed active members of Alphaproteobacteria in all samples and of Sulfobacillus genus in older ones. Also, new active groups such as Actinobacteria and Acidobacterium genus were detected by PAM. The results suggest that changes during the leaching cycle in chemical and physical conditions, such as pH and Fe3+/Fe2+ ion rate, are primary factors shaping the microbial dynamic in the heap.
dc.descriptionThis work was supported in part by FONDEF Proyect D04I1169 from CONICYT and a technological stay in the Centro de Astrobiologia (CSIC-INTA) from CORFO. F. Remonsellez is supported in part by the Technology and Science Bicentennial Program from CONICYT. V. Parro had a 'Ramon y Cajal' contract from the Spanish Ministerio de Ciencia e Innovacion. We thank Alex Echeverria for his help in the phylogenetic tree analysis.
dc.description25
dc.descriptionFONDEF
dc.descriptioncdemerga@ucn.cl
dc.descriptionCONICYT [D04I1169]; CORFO; Spanish Ministerio de Ciencia e Innovacion
dc.description6
dc.descriptionFONDEF
dc.description2
dc.languageENG
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinfo:eu-repo/grantAgreement/Fondef/D04I1169
dc.relationinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relationhttps://doi.org/10.1111/j.1751-7915.2009.00112.x
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleDynamic of active microorganisms inhabiting a bioleaching industrial heap of low-grade copper sulfide ore monitored by real-time pcr and oligonucleotide prokaryotic acidophile microarray
dc.titleMICROBIAL BIOTECHNOLOGY (ONLINE)
dc.titleMICROB BIOTECHNOL
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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