Nitric oxide synthase and changes in oxidative stress levels in embryonic kidney observed in a rabbit model of intrauterine growth restriction

PRENATAL DIAGNOSIS

dc.creatorFigueroa, Horacio
dc.creatorCifuentes, Jorge
dc.creatorLozano, Mauricio
dc.creatorAlvarado, Cristobal
dc.creatorCabezas, Claudia
dc.creatorEixarch, Elisenda
dc.creatorFernández, Ellio
dc.creatorContreras, Luis
dc.creatorIllanes, Sebastián E
dc.creatorHernández-Andrade, Edgar
dc.creatorGratacos, Eduard
dc.creatorIrarrázabal-Muñoz, Carlos Ernesto
dc.date2021-08-23T22:54:46Z
dc.date2022-07-07T02:29:58Z
dc.date2021-08-23T22:54:46Z
dc.date2022-07-07T02:29:58Z
dc.date2016
dc.date.accessioned2023-08-22T01:22:30Z
dc.date.available2023-08-22T01:22:30Z
dc.identifier1151157
dc.identifier1151157
dc.identifierhttps://hdl.handle.net/10533/251479
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8305511
dc.descriptionObjectiveThis work aimed to study the effect of uteroplacental circulation restriction on endothelial kidney damage in a fetal rabbit model. MethodsNew Zealand rabbits were subjected to 40% to 50% of uteroplacental artery ligation at day 25 of pregnancy. After 5days, surviving fetuses were harvested by cesarean section. The gene and protein expressions of selected enzymes associated with nitric oxide production and oxidative stress were analyzed in fetal kidney homogenates. ResultsThe placenta weight (6.060.27, p<0.0319) and fetal body (19.901.03, p<0.0001) were significantly reduced in the uteroplacental circulation restriction group. The kidneys from restricted fetuses presented a mild vascular congestion and glomerular capillary congestion, without inflammation or hypertrophy. We found endothelial nitric oxide synthase phosphorylation inhibition (0.230.13, p<0.012) and arginase-2 (0.290.14, p<0.023) protein induction in fetal kidneys of the circulation restriction group. Finally, the kidneys from circulation-restricted fetuses showed increased inducible nitric oxide synthase messenger RNA (mRNA) (2.680.24, p<0.01) and reduced heme oxygenase-1 mRNA (231.3, p<0.003), with increased reactive oxygen species (1.690.09, p<0.001) and nitrotyrosine protein (1.74 +/- 0.28, p<0.003) levels, without changes in Nox mRNA. ConclusionWe describe significant deregulation of vascular activity and oxidative damage in kidneys of fetal rabbits that have been exposed to restriction of the uterine circulation. (c) 2016 John Wiley & Sons, Ltd.
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.1002/pd.4829
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleNitric oxide synthase and changes in oxidative stress levels in embryonic kidney observed in a rabbit model of intrauterine growth restriction
dc.titlePRENATAL DIAGNOSIS
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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