Trna genes were found in piscirickettsia salmonis 16s-23s rdna spacer region (its)

FEMS MICROBIOLOGY LETTERS;
FEMS Microbiol. Lett.

dc.creatorSANDINO-GARCIA, ANA
dc.creatorCASANOVA, A.
dc.creatorOBREQUE, J.
dc.date2017-04-27T18:50:54Z
dc.date2022-07-07T02:20:06Z
dc.date2017-04-27T18:50:54Z
dc.date2022-07-07T02:20:06Z
dc.date2001
dc.date.accessioned2023-08-21T21:55:29Z
dc.date.available2023-08-21T21:55:29Z
dc.identifier1574-6968
dc.identifierD97I2034
dc.identifierD97I2034
dc.identifierWOS:000167980100003
dc.identifier0378-1097
dc.identifierhttps://hdl.handle.net/10533/197226
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8287274
dc.descriptionPiscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S-23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF-89 and EM-90. Two main ITS amplification products were obtained by PCR using L1 and G1 primers, differing from that described where only one ITS region was Found. By Southern blot, it was established that these two amplification products contained sequences related to P. salmonis ITS. Sequence analysis confirmed that P. salmonis had two ITS regions: ITS A and ITS B. In both isolates, the smaller (ITS B) corresponded to ITS sequences previously described for each one, and the larger (ITS A) were almost the same as their respective ITS B sequences interrupted by an insert which contained two tRNAs genes: tRNA-Ile and rRNA-Ala. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.description0
dc.description10
dc.descriptionFONDEF
dc.descriptionmjashes@lauca.usach.cl
dc.description0
dc.description1
dc.descriptionFONDEF
dc.description197
dc.languageENG
dc.publisherOXFORD UNIV PRESS
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinfo:eu-repo/grantAgreement/Fondef/D97I2034
dc.relationinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relationhttps://doi.org/10.1111/j.1574-6968.2001.tb10576.x
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleTrna genes were found in piscirickettsia salmonis 16s-23s rdna spacer region (its)
dc.titleFEMS MICROBIOLOGY LETTERS
dc.titleFEMS Microbiol. Lett.
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.coverageOXFORD


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