Development of an electrochemical impedimetric immunosensor for Corticotropin Releasing Hormone (CRH) using half-antibody fragments as elements of biorecognition

BIOSENSORS & BIOELECTRONICS

dc.creatorDuran, B G
dc.creatorCastañeda-Parra, Erik Alexander
dc.creatorArmijo-Mancilla, Juan Francisco
dc.date2021-08-23T22:58:43Z
dc.date2022-07-07T14:55:28Z
dc.date2021-08-23T22:58:43Z
dc.date2022-07-07T14:55:28Z
dc.date2019
dc.date.accessioned2023-08-21T20:53:16Z
dc.date.available2023-08-21T20:53:16Z
dc.identifier1150228
dc.identifier1150228
dc.identifierhttps://hdl.handle.net/10533/252390
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8280960
dc.descriptionAn electrochemical impedimetric immunosensor was developed for the detection of the neuropeptide Corticotropin Releasing Hormone (CRH) based on the immobilization of half-antibody fragments on gold nanoparticles (AuNp). Then, the optimal conditions for the obtainment of AuNp through electroplating on a bare gold electrode were studied. The results showed that the obtainment of AuNp at a fixed potential of -0.2 V for 330 s, at 80 degrees C and 2.10-3 mol.L-1 of HAuCl4 generates an adequate nanostructured surface and is a highly reproducible method. Also, the optimal conditions for immobilizing the half-antibody on AuNp were studied. The interaction of the CRH with the recognition layer of the immobilized half-antibody on the nanostructured surface was carried out by incubation at 4 degrees C for 2 h. A dissolution of [Fe(CN)(6)](4)/[Fe(CN)(6)](3-) as a redox probe was used to study the electrochemical responses of the nanostructured surface and the immobilization processes of the half -antibody and detection of CRH, using cyclic voltammetry and electrochemical impedance spectroscopy. An immunosensor was obtained for the specific detection of CRH, within a range of 10.0-80.0 mu g mL(-1), with a limit of detection of 2.7 mu g mL(-1) and a limit of quantification of 9.2 mu g mL(-1) . Additionally, the association constant between the CRH and the immobilized half -antibody was calculated at 1.96.10(5) M-1.
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.1016/j.bios.2019.02.017
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleDevelopment of an electrochemical impedimetric immunosensor for Corticotropin Releasing Hormone (CRH) using half-antibody fragments as elements of biorecognition
dc.titleBIOSENSORS & BIOELECTRONICS
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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