In Vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration Using Sensory Neurons from Dorsal Root Ganglia

Methods in Molecular Biology

dc.creatorLópez-Leal, Rodrigo
dc.creatorDíaz, Paula
dc.creatorCourt-Goldsmith, Felipe Alfredo
dc.date2021-08-23T22:51:22Z
dc.date2022-07-08T20:28:59Z
dc.date2021-08-23T22:51:22Z
dc.date2022-07-08T20:28:59Z
dc.date2018
dc.date.accessioned2023-08-21T20:48:38Z
dc.date.available2023-08-21T20:48:38Z
dc.identifier1150766
dc.identifier1150766
dc.identifierhttps://hdl.handle.net/10533/250770
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8280481
dc.descriptionSensory neurons from dorsal root ganglion efficiently regenerate after peripheral nerve injuries. These neurons are widely used as a model system to study degenerative mechanisms of the soma and axons, as well as regenerative axonal growth in the peripheral nervous system. This chapter describes techniques associated to the study of axonal degeneration and regeneration using explant cultures of dorsal root ganglion sensory neurons in vitro in the presence or absence of Schwann cells. Schwann cells are extremely important due to their involvement in tissue clearance during axonal degeneration as well as their known pro-regenerative effect during regeneration in the peripheral nervous system. We describe methods to induce and study axonal degeneration triggered by axotomy (mechanical separation of the axon from its soma) and treatment with vinblastine (which blocks axonal transport), which constitute clinically relevant mechanical and toxic models of axonal degeneration. In addition, we describe three different methods to evaluate axonal regeneration using quantitative methods. These protocols constitute a valuable tool to analyze in vitro mechanisms associated to axonal degeneration and regeneration of sensory neurons and the role of Schwann cells in these processes. Keywords. Author Keywords:Dorsal root ganglion; Sensory neurons; Schwann cell; Axonal degeneration; Axonal regeneration; Axonal growth. KeyWords Plus:PERIPHERAL-NERVE REGENERATION; TRANSPORT
dc.descriptionRegular 2015
dc.descriptionFONDECYT
dc.descriptionFONDECYT
dc.languageeng
dc.relationhandle/10533/111557
dc.relationhandle/10533/111541
dc.relationhandle/10533/108045
dc.relationhttps://doi.org/10.1007/978-1-4939-7649-2_16
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsinfo:eu-repo/semantics/article
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleIn Vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration Using Sensory Neurons from Dorsal Root Ganglia
dc.titleMethods in Molecular Biology
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/publishedVersion


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