Sodium/proton exchanger isoform 1 regulates intracellular pH and cell proliferation in human ovarian cancer

Biochimica Et Biophysica Acta. Molecular Basis Of Disease

dc.creatorSanhueza, Carlos
dc.creatorAraos, Joaquín
dc.creatorNaranjo, Luciano
dc.creatorToledo, Fernando
dc.creatorBeltrán, Ana R
dc.creatorRamírez, Marco A
dc.creatorGutierrez, Jaime
dc.creatorPardo, Fabián
dc.creatorLeiva, Andrea
dc.creatorSobrevia-Luarte, Luis Alberto
dc.date2019-06-25T21:47:30Z
dc.date2022-07-07T21:48:07Z
dc.date2019-06-25T21:47:30Z
dc.date2022-07-07T21:48:07Z
dc.date2017
dc.date.accessioned2023-08-21T20:39:13Z
dc.date.available2023-08-21T20:39:13Z
dc.identifier1150377
dc.identifier1150377
dc.identifierhttps://hdl.handle.net/10533/236138
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8279507
dc.descriptionCancer cells generate protons (H+) that are extruded to the extracellular medium mainly via the Na+/H+ exchanger 1 (NHE1), which regulates intracellular pH (pHi) and cell proliferation. In primary cultures of human ascites-derived ovarian cancer cells (haOC) we assayed whether NHE1 was required for pHi modulation and cell proliferation. Human ovary expresses NHE1, which is higher in haOC and A2780 (ovarian cancer cells) compared with HOSE cells (normal ovarian cells). Basal pHi and pHi recovery (following a NH4Cl pulse) was higher in haOC and A2780, compared with HOSE cells. Zoniporide (NHE1 inhibitor) caused intracellular acidification and pHi recovery was independent of intracellular buffer capacity, but reduced in NHE1 knockdown A2780 cells. Zoniporide reduced the maximal proliferation capacity, cell number, thymidine incorporation, and ki67 (marker of proliferation) fluorescence in haOC cells. SLC9A1 (for NHE1) amplification associated with lower overall patient survival. In conclusion, NHE1 is expressed in human ovarian cancer where it has a pro-proliferative role. Increased NHE1 expression and activity constitute an unfavourable prognostic factor in these patients. (C) 2016 Elsevier B.V. All rights reserved. Keywords. Author Keywords:NHE1; pHi; Human; Ovarian cancer; Cell proliferation
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinfo:eu-repo/grantAgreement//1150377
dc.relationinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.relationhttps://www.sciencedirect.com/science/article/pii/S0925443916302575?via%3Dihub
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.titleSodium/proton exchanger isoform 1 regulates intracellular pH and cell proliferation in human ovarian cancer
dc.titleBiochimica Et Biophysica Acta. Molecular Basis Of Disease
dc.typeArticulo
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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