Six of the samples were debittered; seven were left in their bitter, as harvested, state. To debitter the samples, they were soaked for 12 hours, boiled for 40 min and washed in water for 72 hours. The debittered bean was then dried in a stove at 50ºC, with forced air, until the moisture content was 10%. Then the beans (bitter and debittered) were milled in a disc mill with a mesh opening of 1 mm and stored in sealed glass jars until analysis. The oil was extracted from a 50 g sample of milled bean (20 mesh) by refluxing for 6 h with 125 mL nhexane (ACS) in a Soxhlet extractor. The solvent was evaporated under reduced pressure, yielding the bitter oil from the beans processed as harvested and debittered oil from debittered beans. Esterfication of the oil was carried out on 50 mg samples of the extracted oil, to which was added 1 ml of 0.5 M KOH in methanol.