Evaluación de una ADN polimerasa Bst_LF de producción local en ensayos LAMP para la detección de Neospora caninum en muestras de bovinos (Bos taurus)
Fecha
2023-08-02Autor
Sigüenza Jiménez, Estefanía Tatiana
Institución
Resumen
Neospora caninum is an intracellular parasite of great importance in cattle breeding due to the
great economic losses, both productive and reproductive. In our country, molecular techniques
applicable to diagnosis are not available to most farmers due to the costs and time required
to obtain the results, which prevents an accurate diagnosis of the disease. The objective of
this study is to evaluate the use of a DNA polymerase Bst_LF enzyme produced in the
Molecular Biology Laboratory of the Faculty of Agropecuary Sciences of the University of
Cuenca in a LAMP assay, for the detection of N. caninum genetic material in biological
samples of bovine (Bos taurus), using a commercial enzyme as a reference. We used 50 fetal
brain tissue samples and 50 milk samples. The results obtained in the milk samples with the
two enzymes were 100 % negative, therefore it was not possible to detect DNA of the
pathogen. In the brain samples, 40% of positive samples were identified with the commercial
enzyme and 26% of positive samples with the local enzyme, there being a numerical
difference but no significant differences with McNemar's analysis. With McNemar's statistical
test there were no significant differences between both enzymes (p = 0.0654), therefore the
local enzyme Bst_LF shows the same capacity for the detection of N. caninum genetic material
in bovine fetal brain tissue compared to that of the commercial enzyme Bsm.