Optimización de ensayos de ELISA indirecto y Dot Blot para la detección de anticuerpos anti- Toxoplasma gondii en felinos (Felis catus domesticus) con las proteínas recombinantes TgSAG2 y TgGRA7

Abstract

Toxoplasmosis is caused by the single-celled parasite Toxoplasma gondii, whose definitive hosts are domestic cats. This infection tends to show some clinical symptoms. In some cases, it might produces some digestive, neuronal, and reproductive disorders. T. gondii infection might be acquired through contaminated water or food containing oocysts, therefore, its diagnosis is important, since in the cat the parasite reproduces sexually, thus allowing a greater diversity of genes in T. gondii and in its next generations. Several studies suggest promising alternatives to identify T gondii antigens in cats using recombinant proteins in tests like indirect ELISA and Dot Blot, this research reaches to optimize that tests to detect antibodies anti-T. gondii. In our serologic samples from domestic cats, using recombinant proteins TgSAG2 and TgSAG7 from T gondii. It obtained great results,the results in indirect ELISA using as the protocol of the combination of the antigen TgSAG2Multisorp plaque, BSA, and as substrate ABTS. For the protocol of Dot Blot, the best combination was with the antigen TgSAG2 membrane PVDF. Substrate DAB and dilution in BSA and blocking with BSA. With respect to the test there were no significant differences between the test of indirect ELISA, and Dot Blot, showing that both methods are suitable for the diagnosis of infection by T gondii in a short period of time and with a minimum amount of money.