| dc.creator | MARCO ANTONIO TORRES CASTRO | |
| dc.date | 2016-09-30 | |
| dc.date.accessioned | 2023-07-25T13:56:30Z | |
| dc.date.available | 2023-07-25T13:56:30Z | |
| dc.identifier | http://redi.uady.mx:8080/handle/123456789/3733 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7797356 | |
| dc.description | Toxoplasma gondii is an obligate intracellular parasite recognized as the
etiological agent of Toxoplasmosis, a zoonotic disease endemic in different
countries worldwide. In Mexico, the disease is recognized as a human and
animal health problem. T. gondii sporozoites have the capacity of been
released from the gut lumen and infect several tissues, including kidneys.
Rodents have been identified as intermediate hosts of T. gondii; nevertheless,
no previous reports exist in Yucatan, Mexico, about their role in the infectious
cycle. A total of 187 specimens (130 Mus musculus and 57 Rattus rattus)
were studied. Total DNA was extracted from renal tissue and was utilized
in the identification of T. gondii with the end-point Polymerase Chain
Reaction (PCR) method. Six positive products were purified and sequenced
in order to confirm and validate the results through the BLAST Sequence
Analysis Tool. The PCR provided a global positivity of 5.34% (10/187). The
BLAST analysis found a direct homology for T. gondii with identities and
coverage of 96-98% and 98-100%, respectively. Our results confirm the role
of M. musculus and R. rattus in the biological cycle of T. gondii and offer
knowledge of the parasite transmission in intermediary hosts of the region. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.source | urn:issn:2258-0646 | |
| dc.subject | info:eu-repo/classification/cti/6 | |
| dc.subject | Toxoplasma gondii | |
| dc.subject | Rattus rattus | |
| dc.subject | Mus musculus | |
| dc.subject | Kidneys | |
| dc.title | First molecular evidence of Toxoplasma gondii in synanthropic rodents (Mus musculus and Rattus rattus) captured in Yucatan, Mexico | |
| dc.type | info:eu-repo/semantics/article | |
| dc.coverage | Generación de conocimiento | |
| dc.audience | researchers | |
