Effects on Capsicum annuum Plants Colonized with Trichoderma atroviride P. Karst Strains Genetically Modified in Taswo1, a Gene Coding for a Protein with Expansin-like Activity

dc.creatorRICARDO SANCHEZ CRUZ
dc.creatorKARINA ATRIZTAN HERNANDEZ
dc.creatorMARIA DEL RAYO SANCHEZ CARBENTE
dc.creatorVERONICA LIRA RUAN
dc.creatorRAMON ALBERTO BATISTA GARCIA
dc.creatorJorge Luis Folch Mallol
dc.date2021
dc.date.accessioned2023-07-25T12:47:44Z
dc.date.available2023-07-25T12:47:44Z
dc.identifier2223-7747
dc.identifierhttp://riaa.uaem.mx/handle/20.500.12055/3507
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7792566
dc.descriptionHere, we analyzed the effects on Capsicum annuum plants of Trichoderma atroviride P. Karst strains altered in the expression of SWOLLENIN (SWO1), a protein with amorphogenic activity on plant cell wall components. Strains of T. atroviride that overexpressed the Taswo1 gene were constructed as well as deletion mutants. A novel, cheap and accurate method for assessing root colonization was developed. Colonization assays showed that the Taswo1 overexpressing strains invaded the host root better than the WT, resulting in a stronger plant growth-promoting effect. The expression of plant defense marker genes for both the systemic acquired resistance and induced systemic resistance pathways was enhanced in plants inoculated with Taswo1 overexpressing strains, while inoculation with deletion mutant strains resulted in a similar level of expression to that observed upon inoculation with the wild-type strain. Response to pathogen infection was also enhanced in the plants inoculated with the Taswo1 overexpressing strains, and surprisingly, an intermediate level of protection was achieved with the mutant strains. Tolerance to abiotic stresses was also higher in plants inoculated with the Taswo1 overexpressing strains but was similar in plants inoculated with the wild-type or the mutant strains. Compatible osmolyte production in drought conditions was studied. This study may contribute to improving Trichoderma biocontrol and biofertilization abilities
dc.formatpdf
dc.languageeng
dc.publisherMDPI Open Access Journals
dc.relationPlants
dc.relation9
dc.relation10
dc.relationhttps://www.mdpi.com/2223-7747/10/9/1919
dc.relationinfo:eu-repo/semantics/altIdentifier/issn/2223-7747
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/4.0
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectTrichoderma, phytopathogen resistance, stress tolerance, SWOLLENIN
dc.titleEffects on Capsicum annuum Plants Colonized with Trichoderma atroviride P. Karst Strains Genetically Modified in Taswo1, a Gene Coding for a Protein with Expansin-like Activity
dc.typeinfo:eu-repo/semantics/article
dc.coverageMEX
dc.audienceresearchers


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