Curcumin Stimulates the Overexpression of Virulence Factors in Salmonella enterica Serovar Typhimurium: In Vitro and Animal Model Studies

dc.creatorZermeño Ruíz, Martín
dc.creatorRangel Castañeda, Itzia A.
dc.creatorSuárez Rico, Daniel Osmar
dc.creatorHernández Hernández, Leonardo
dc.creatorCortés Zárate, Rafael
dc.creatorHernández Hernández, José M.
dc.creatorCamargo Hernández, Gabriela
dc.creatorCastillo Romero, Araceli
dc.date2023-02-08T23:33:06Z
dc.date2023-02-08T23:33:06Z
dc.date2022-09
dc.date.accessioned2023-07-21T21:46:13Z
dc.date.available2023-07-21T21:46:13Z
dc.identifier: Zermeño-Ruiz, M.; Rangel-Castañeda, I.A.; Suárez-Rico, D.O.; Hernández-Hernández, L.; Cortés-Zárate, R.; Hernández-Hernández, J.M.; Camargo-Hernández, G.; Castillo-Romero, A. Curcumin Stimulates the Overexpression of Virulence Factors in Salmonella enterica Serovar Typhimurium: In Vitro and Animal Model Studies. Antibiotics 2022, 11, 1230. https:// doi.org/10.3390/antibiotics11091230
dc.identifier2079-6382
dc.identifierhttps://doi.org/10.3390/antibiotics11091230
dc.identifierhttp://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1465
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7752933
dc.descriptionArtículo
dc.descriptionAbstract Salmonella spp. is one of the most common food poisoning pathogens and the main cause of diarrheal diseases in humans in developing countries. The increased Salmonella resistance to antimicrobials has led to the search for new alternatives, including natural compounds such as curcumin, which has already demonstrated a bactericidal effect; however, in Gram-negatives, there is much controversy about this effect, as it is highly variable. In this study, we aimed to verify the antibacterial activity of curcumin against the Salmonella enterica serovar Typhimurium growth rate, virulence, and pathogenicity. The strain was exposed to 110, 220 or 330 µg/mL curcumin, and by complementary methods (spectrophotometric, pour plate and MTT assays), we determined its antibacterial activity. To elucidate whether curcumin regulates the expression of virulence genes, Salmonella invA, fliC and siiE genes were investigated by quantitative real-time reverse transcription (qRT-PCR). Furthermore, to explore the effect of curcumin on the pathogenesis process in vivo, a Caenorhabditis elegans infection model was employed. No antibacterial activity was observed, even at higher concentrations of curcumin. All concentrations of curcumin caused overgrowth (35–69%) and increased the pathogenicity of the bacterial strain through the overexpression of virulence factors. The latter coincided with a significant reduction in both the lifespan and survival time of C. elegans when fed with curcumin-treated bacteria. Our data provide relevant information that may support the selective antibacterial effects of curcumin to reconsider the indiscriminate use of this phytochemical, especially in outbreaks of pathogenic Gram-negative bacteria.
dc.languageen
dc.publisherMDPI. International Journal of Environmental Research and Public Health
dc.relationAntibiotics;2022, 11, 1230
dc.subjectSalmonella enterica serovar Typhimurium
dc.subjectcurcumin
dc.subjectantibacterial activity
dc.subjectpathogenicity
dc.subjectC. elegans
dc.titleCurcumin Stimulates the Overexpression of Virulence Factors in Salmonella enterica Serovar Typhimurium: In Vitro and Animal Model Studies
dc.typeArticle


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