17β-estradiol modulates the expression of hormonal receptors on THP-1 T. gondii-infected macrophages and monocytes in an AKT and ERK-dependent manner

dc.creatorPereira Suarez, Ana Laura
dc.creatorGalván Ramírez, María de la Luz
dc.creatorRodríguez Pérez, Laura Rocío
dc.creatorLópez Pulido, Edgar Iván
dc.creatorHernández Silva, Christian David
dc.creatorRamírez López, Inocencia Guadalupe
dc.creatorMorales Amaya, Grecia Viridiana
dc.creatorLópez Cabrera, Luis David
dc.creatorMuñoz Valle, José Francisco
dc.creatorRamírez de Arellano, Adrián
dc.date2022-07-04T19:30:48Z
dc.date2022-07-04T19:30:48Z
dc.date2022-01
dc.date.accessioned2023-07-21T21:45:33Z
dc.date.available2023-07-21T21:45:33Z
dc.identifierPereira-Suárez A.L., Galván-Ramírez M.L., Rodríguez-Pérez L.R., López-Pulido E.I., Hernández-Silva C.D., Ramírez-López I.G., Morales Amaya G.V., Lopez Cabrera L.D., Muñoz-Valle J.F., Ramírez-de-Arellano A. (2022). 17β-estradiol modulates the expression of hormonal receptors on THP-1 T. gondii-infected macrophages and monocytes in an AKT and ERK-dependent manner. Molecular and Biochemical Parasitology, Volume 247, 111433. DOI: https://doi.org/10.1016/j.molbiopara.2021.111433
dc.identifier0166-6851
dc.identifierhttps://doi.org/10.1016/j.molbiopara.2021.111433
dc.identifierhttp://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1373
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7752844
dc.descriptionArtículo
dc.descriptionAbstract Toxoplasma gondii (T. gondii) is a parasite common in pregnancy. Monocytes and macrophages are a significant immunologic barrier against T. gondii by boosting up inflammation. This outcome is highly regulated by signaling pathways such as MAPK (ERK1/2) and PI3K (AKT), necessary in cell growth and proliferation. It may be associated with the hormonal receptors’ modulation by T. gondii (Estrogen Receptor (ER)-α, ERβ, G Protein-coupled ER (GPER), and Prolactin Receptor (PRLR)), as previously reported by our research group. 17β-estradiol also activates MAPK and PI3K; however, its combined effect in THP-1 monocytes and macrophages, infected with T. gondii, has not yet been evaluated. This study aimed to evaluate the combined effect of 17β-estradiol in the activation of signaling pathways using a model of THP-1 monocytes and macrophages infected with T. gondii. THP-1 monocytes were cultured and differentiated into macrophages. Inhibition of AKT and ERK1/2 was performed with specific inhibitors. Stimuli were performed with 17β-estradiol (10 nM), T. gondii (20,000 tachyzoites), and both conditions for 48 h. Proteins were extracted and quantified, and Western Blot assays were performed. 17β-estradiol performed activation of ERK1/2 and AKT in T. gondii-infected macrophages. 17β-estradiol modulated the expression of hormonal receptors in infected cells: increases the PRLR and PrgR in T. gondii-infected macrophages and decreases the PRLR and ERα in T. gondii-infected monocytes. As for GPER, its expression is abolished by T. gondii, and 17β-estradiol cannot restore it. Finally, the blockage of ERK and AKT pathways modified the expression of hormonal receptors. In conclusion, 17β-estradiol modifies the receptors of T. gondii-infected THP1 macrophages and monocytes in an ERK/AKT dependent manner.
dc.languageen
dc.publisherElsevier - Science Direct
dc.relationMolecular & Biochemical Parasitology;247 (2022) 111433
dc.subjectT. gondii
dc.subject17β-estradiol
dc.subjectAKT
dc.subjectERK
dc.subjectTHP-1
dc.title17β-estradiol modulates the expression of hormonal receptors on THP-1 T. gondii-infected macrophages and monocytes in an AKT and ERK-dependent manner
dc.typeArticle


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