Antinociceptive effects of Laelia anceps Lindl. and Cyrtopodium macrobulbon (Lex.) G.A. Romero & Carnevali, and comparative evaluation of their metabolomic profiles

dc.creatorYáñez Barrientos, Eunice
dc.creatorGonzález Ibarra, Alan Alexander
dc.creatorWrobel, Kazimierz
dc.creatorWrobel, Katarzyna
dc.creatorCorrales Escobosa, Alma Rosa
dc.creatorAlonso Castro, Angel Josabad
dc.creatorCarranza Álvarez, Candy
dc.creatorPonce Hernández, Amauri
dc.creatorIsiordia Espinoza, Mario Alberto
dc.creatorOrtiz Andrade, Rolffy
dc.creatorTorres Gutiérrez, Karen Lizbeth
dc.creatorLuna Rubio, Sarahí
dc.creatorZapata Morales, Juan Ramón
dc.date2022-03-10T16:47:16Z
dc.date2022-03-10T16:47:16Z
dc.date2022-03
dc.date.accessioned2023-07-21T21:45:02Z
dc.date.available2023-07-21T21:45:02Z
dc.identifierYáñez-Barrientos E., González-Ibarra A.A., Wrobel K., Wrobel K., Corrales-Escobosa A.R., Alonso-Castro A.J., Carranza-Álvarez C., Ponce-Hernández A., Isiordia-Espinoza M.A., Ortiz-Andrade R, Torres-Gutiérrez K.L., Luna-Rubio S. & Zapata-Morales J.R. (2022). Antinociceptive effects of Laelia anceps Lindl. and Cyrtopodium macrobulbon (Lex.) G.A. Romero & Carnevali, and comparative evaluation of their metabolomic profiles. Journal of Ethnopharmacology, art. 115172. ISSN 0378-8741. DOI: https://doi.org/10.1016/j.jep.2022.115172.
dc.identifier0378-8741
dc.identifierhttps://doi.org/10.1016/j.jep.2022.115172.
dc.identifierhttp://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1302
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7752778
dc.descriptionArtículo
dc.descriptionAbstract: Ethnopharmacological relevance Laelia anceps and Cyrtopodium macrobulbon are two orchids used in Mexican traditional medicine for treating pain. Aim of the study The individual antinociceptive activity of ethanol extracts from the roots of Laelia anceps (LAE) and Cyrtopodium macrobulbon (CME) was evaluated, and their metabolomic profiles were comparatively evaluated. The antinociceptive activity of CME and naproxen combination (1:1) was also addressed. Materials and methods The antinociceptive actions of LAE and CME were examined using three nociceptive tests. The combination of CME with naproxen was evaluated in the acetic acid test using isobologram analysis. Metabolomic analysis was performed using capillary reversed phase liquid chromatography–electrospray ionization-high resolution mass spectrometry and the MS-DIAL 4.70 software was used for data analysis and statistics. Results LAE (ED50 = 48.4 mg/kg) and CME (ED50 = 17.8 mg/kg) showed antinociceptive activity in the acetic acid test. Pre-treatment with L-NAME reverted the antinociceptive effects of LAE and CME in the acetic acid test. LAE (ED50 = 97 mg/kg) and CME (ED50 = 29 mg/kg) also induced antinociceptive activity in the second phase of the formalin test. The combination of CME with naproxen induced synergistic (interaction index = 0.434) antinociceptive effects (ED50 = 10.6 mg/kg). Overall, 156 compounds allocated in 97 different ontologies were found to be differentially expressed in the two orchids; among them, 125 compounds corresponded to LAE and 31 to CME. Three phenanthrene derivatives annotated in CME might be associated with its antinociceptive activity. Conclusion LAE and CME induced antinociceptive activity with the possible participation of the nitric oxide pathway. CME in combination with naproxen synergistically produces antinociceptive effects in the acetic acid test. The untargeted metabolomic analysis allowed for annotation of several compounds potentially involved in the therapeutic potential of two plants; among them, three phenanthrene derivatives might contribute to the observed antinociceptive activity.
dc.languageen
dc.publisherElsevier
dc.relationJournal of Ethnopharmacology;2022
dc.subjectlaelia anceps
dc.subjectcyrtopodium macrobulbon
dc.subjectnitric oxide
dc.subjectantinociceptive
dc.titleAntinociceptive effects of Laelia anceps Lindl. and Cyrtopodium macrobulbon (Lex.) G.A. Romero & Carnevali, and comparative evaluation of their metabolomic profiles
dc.typeArticle


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