IRS-1 pY612 and Akt-1/PKB pT308 Phosphorylation and Antiinflammatory Effect of Diindolylmethane in Adipocytes Cocultured with Macrophages

dc.creatorLópez Vázquez, Alfonso
dc.creatorGarcía Bañuelos, Jesús Javier
dc.creatorGonzález Garibay, Angélica Sofía
dc.creatorUrzúa Lozano, Pedro Ernesto
dc.creatorDel Toro Arreola, Susana
dc.creatorBueno Topete, Miriam Ruth
dc.creatorSánchez Enríquez, Sergio
dc.creatorMuñoz Valle, José Francisco
dc.creatorJave Suárez, Luis Felipe
dc.creatorArmendáriz-Borunda, Juan
dc.creatorBastidas Ramírez, Blanca Estela
dc.date2022-02-15T19:42:16Z
dc.date2022-02-15T19:42:16Z
dc.date2017-12
dc.date.accessioned2023-07-21T21:44:56Z
dc.date.available2023-07-21T21:44:56Z
dc.identifierLopez-Vazquez A. Garcia-Banuelos JJ *, Gonzalez-Garibay AS, Urzua-Lozano PE, Del Toro-Arreola S, Bueno-Topete MR, Sanchez-Enriquez S, Munoz-Valle JF, Jave-Suarez LF, Armendariz-Borunda J and Bastidas-RamireZ BE. (2017). IRS-1 pY612 and Akt-1/PKB pT308 Phosphorylation and Antiinflammatory Effect of Diindolylmethane in Adipocytes Cocultured with Macrophages. Medicinal Chemistry, Vol. 13, Issue 8. 727-733 pp. DOI: 10.2174/1573406413666170922095011
dc.identifier1875-533X online
dc.identifier0929-8673 print
dc.identifierDOI: 10.2174/1573406413666170922095011
dc.identifierhttp://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1287
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7752763
dc.descriptionArtículo
dc.descriptionAbstract: Background: 3,3’-Diindolylmethane (DIM) is a condensation product of indole-3-carbinol, a glucosinolate naturally occurring in Brassica genus vegetables. The antiinflammatory properties of DIM through the inhibition of NF-B, as well as its ameliorating effects on glucose tolerance and hyperglicemic states, have been described. A subclinical proinflammatory profile resultant from the interaction of adipocytes and macrophages has been reported in obesity, affecting the insulin signaling pathway, contributing to insulin resistance. Objective: The aim of this study was to evaluate the effect of DIM on proinflammatory cytokines and phosphorylation of IRS-1 pY612 and Akt-1/PKB pT308 in an obesity-induced inflammation model. Methods: Differentiated 3T3-L1 adipocytes were co-cultured with RAW 264.7 macrophages and exposed to 20 M, 40 M and 60 M DIM for 24 h followed by 100 nM insulin for 20 min. MCP-1, IL-6 and TNF were quantified in the supernatant through individual ELISAs. Adipocyte lysates were used to determine the relative expression of the proinflammatory mediators by qPCR, and the phosphorylation of IRS-1 pY612 and Akt-1/PKB pT308 proteins by western blot analysis. Results: DIM significantly (p<0.05) reduced the production and mRNA expression of MCP-1, IL-6, and TNF in a DIM concentration dependent manner, concomitantly increasing the abundance of IRS-1 pY612 and Akt-1/PKB pT308. Conclusion: Our results suggest that DIM influences the insulin transduction pathway by exerting an antiinflammatory effect. The potential therapeutic benefits of DIM in the treatment of glucose metabolic disorders deserve further studies.
dc.languageen
dc.publisherBentham Science
dc.relationMedicinal Chemistry;Volume 13, Issue 8 , December 2017
dc.subjectdiabetes mellitus type 2
dc.subjectglucose
dc.subjectinflammation
dc.subjectinsulin resistance
dc.subjectinsulin signaling pathway
dc.subjectobesity
dc.subjectphytochemicals
dc.titleIRS-1 pY612 and Akt-1/PKB pT308 Phosphorylation and Antiinflammatory Effect of Diindolylmethane in Adipocytes Cocultured with Macrophages
dc.typeArticle


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