"In this dissertation, a biosensing system targeting pathogen-related analytes was developed. The biosensing system comprises 96 microwell plates coated with graphene oxide, which performs as quencher of fluorescence from a specific immunoprobe. The fluorescence of the immunoprobes that do not experiment immunoreactions (antibody-antigen) are deactivated by graphene oxide via non-radiative energy transfer, whereas those immunoprobes undergoing immunoreactions preserve its photoluminescence due to the distance and the low affinity between the immunocomplex and the graphene oxide-coated surface. Additionally, based on the same biosensing strategy, it was developed a paper-based disposable test for bacterial vaginosis detection."