dc.contributor | Mata Gómez, Marco Arnulfo | |
dc.contributor | School of Engineering and Sciences | |
dc.contributor | Luna Vital, Diego Armando | |
dc.contributor | Sánchez, Mirna Lorena | |
dc.contributor | González Valdez, José Guillermo | |
dc.contributor | Ibarra Herrera, Celeste Concepción | |
dc.contributor | Campus Monterrey | |
dc.contributor | puelquio/mscuervo | |
dc.creator | MATA GOMEZ, MARCO ARNULFO; 207149 | |
dc.creator | Pérez Rodríguez, Elizabeth | |
dc.date.accessioned | 2023-06-21T16:11:29Z | |
dc.date.accessioned | 2023-07-19T19:20:52Z | |
dc.date.available | 2023-06-21T16:11:29Z | |
dc.date.available | 2023-07-19T19:20:52Z | |
dc.date.created | 2023-06-21T16:11:29Z | |
dc.date.issued | 2021-01-01 | |
dc.identifier | Pérez Rodríguez, E. (2021). Fabrication of a guanidine ligand-based anion exchange monolithic stationary phase in a 3D printed polypropylene housing for protein chromatography [Unpublished master's thesis]. Instituto Tecnológico y de Estudios Superiores de Monterrey. Recuperado de: https://hdl.handle.net/11285/650925 | |
dc.identifier | https://hdl.handle.net/11285/650925 | |
dc.identifier | https://orcid.org/0000-0002-1886-3686 | |
dc.identifier | 1048547 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7715982 | |
dc.description.abstract | A monolithic anion exchange support is synthesized based on the incorporation of γ -guanidinobutyric acid an alkaloid with three resonant amino groups- as ligand onto a poly(EDMA-co-GMA) monolith. The created monolithic anion exchange support is referred as M-Gnd. Monolith was synthesized in a one-step polymerization reaction in a designed and printed polypropylene housing within a 5x20mm i.d. channel. Functionalization of the poly-methacrylate monolith with γ -guanidinobutyric acid was done by Shift-based method using diethylenediamine as a spacer. Homogeneous surface morphology was appreciated by SEM image, and FTIR analysis confirmed the presence of ligand. Highest value of Dynamic binding capacity (12.7mg BSA/mL monolith) was obtained at 0.5 mL/min when a 2 mg/mL protein concentration was used. The estimated ligand for M-Gnd was 1.23 mmol/g dry support. The M-Gnd support allowed to purity a red fluorescent protein up to 75%, presenting a high efficiency (N and HETP values). The characterized M-Gnd showed positive results as anion exchange chromatography support for protein purification. However, the functionalization of γ-guanidinobutyric acid can be improved to have a comparable chromatographic performance to commercial anion exchange monolith. | |
dc.language | eng | |
dc.publisher | Instituto Tecnológico y de Estudios Superiores de Monterrey | |
dc.relation | draft | |
dc.relation | REPOSITORIO NACIONAL CONACYT | |
dc.rights | http://creativecommons.org/licenses/by/4.0 | |
dc.rights | openAccess | |
dc.title | Fabrication of a guanidine ligand-based anion exchange monolithic stationary phase in a 3D printed polypropylene housing for protein chromatography | |
dc.type | Trabajo de grado, Maestría / master Degree Work | |