dc.contributorMata Gómez, Marco Arnulfo
dc.contributorSchool of Engineering and Sciences
dc.contributorLuna Vital, Diego Armando
dc.contributorSánchez, Mirna Lorena
dc.contributorGonzález Valdez, José Guillermo
dc.contributorIbarra Herrera, Celeste Concepción
dc.contributorCampus Monterrey
dc.contributorpuelquio/mscuervo
dc.creatorMATA GOMEZ, MARCO ARNULFO; 207149
dc.creatorPérez Rodríguez, Elizabeth
dc.date.accessioned2023-06-21T16:11:29Z
dc.date.accessioned2023-07-19T19:20:52Z
dc.date.available2023-06-21T16:11:29Z
dc.date.available2023-07-19T19:20:52Z
dc.date.created2023-06-21T16:11:29Z
dc.date.issued2021-01-01
dc.identifierPérez Rodríguez, E. (2021). Fabrication of a guanidine ligand-based anion exchange monolithic stationary phase in a 3D printed polypropylene housing for protein chromatography [Unpublished master's thesis]. Instituto Tecnológico y de Estudios Superiores de Monterrey. Recuperado de: https://hdl.handle.net/11285/650925
dc.identifierhttps://hdl.handle.net/11285/650925
dc.identifierhttps://orcid.org/0000-0002-1886-3686
dc.identifier1048547
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7715982
dc.description.abstractA monolithic anion exchange support is synthesized based on the incorporation of γ -guanidinobutyric acid an alkaloid with three resonant amino groups- as ligand onto a poly(EDMA-co-GMA) monolith. The created monolithic anion exchange support is referred as M-Gnd. Monolith was synthesized in a one-step polymerization reaction in a designed and printed polypropylene housing within a 5x20mm i.d. channel. Functionalization of the poly-methacrylate monolith with γ -guanidinobutyric acid was done by Shift-based method using diethylenediamine as a spacer. Homogeneous surface morphology was appreciated by SEM image, and FTIR analysis confirmed the presence of ligand. Highest value of Dynamic binding capacity (12.7mg BSA/mL monolith) was obtained at 0.5 mL/min when a 2 mg/mL protein concentration was used. The estimated ligand for M-Gnd was 1.23 mmol/g dry support. The M-Gnd support allowed to purity a red fluorescent protein up to 75%, presenting a high efficiency (N and HETP values). The characterized M-Gnd showed positive results as anion exchange chromatography support for protein purification. However, the functionalization of γ-guanidinobutyric acid can be improved to have a comparable chromatographic performance to commercial anion exchange monolith.
dc.languageeng
dc.publisherInstituto Tecnológico y de Estudios Superiores de Monterrey
dc.relationdraft
dc.relationREPOSITORIO NACIONAL CONACYT
dc.rightshttp://creativecommons.org/licenses/by/4.0
dc.rightsopenAccess
dc.titleFabrication of a guanidine ligand-based anion exchange monolithic stationary phase in a 3D printed polypropylene housing for protein chromatography
dc.typeTrabajo de grado, Maestría / master Degree Work


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