Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

dc.contributorSpringer
dc.creatorMedina Rivero, Emilio
dc.creatorBalderas Hernández, Víctor Emmanuel
dc.creatorOrdoñez Acevedo, Leandro Gabriel
dc.creatorPaz Maldonado, Luz María Teresita
dc.creatorBarba de la Rosa, Ana Paulina
dc.creatorDe León Rodríguez, Antonio
dc.date2018-06-07T20:16:38Z
dc.date2018-06-07T20:16:38Z
dc.date2007-09
dc.date.accessioned2023-07-17T22:05:22Z
dc.date.available2023-07-17T22:05:22Z
dc.identifierMedina-Rivero, E., Balderas-Hernández, V.E., Ordoñez-Acevedo, L.G. et al. Biotechnol Lett (2007) 29: 1369. https://doi.org/10.1007/s10529-007-9395-5
dc.identifierhttp://hdl.handle.net/11627/3844
dc.identifierhttps://doi.org/10.1007/s10529-007-9395-5
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7544363
dc.description"Production of periplasmic human interferon-γ (hINF-γ) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-γ was processed and the protein was transported to periplasm. Up to 30.1% of hINF-γ was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction."
dc.formatapplication/pdf
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsAcceso Abierto
dc.subjectPeriplasm
dc.subjectSignal peptide
dc.subjectTat pathway
dc.subjectTherapeutic protein
dc.subjectCIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
dc.titleModified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-γ but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli
dc.typearticle


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