A germin-like protein gene (CchGLP) of Capsicum chinense Jacq. is induced during incompatible interactions and displays Mn-superoxide dismutase activity

dc.contributorMDPI AG
dc.creatorLéon Galvan, Ma Fabiola
dc.creatorJoaquín Ramos, Ahuizotl de Jesús
dc.creatorTorres Pacheco, Irineo
dc.creatorBarba de la Rosa, Ana Paulina
dc.creatorGuevara Olvera, Lorenzo
dc.creatorGonzález Chavira, Mario Martín
dc.creatorOcampo Velázquez, Rosalía Virginia
dc.creatorRico García, Enrique
dc.creatorGuevara González, Ramón Gerardo
dc.creatorOcampo Velázquez, Rosalía Virginia
dc.date2018-03-23T23:59:35Z
dc.date2018-03-23T23:59:35Z
dc.date2011-10
dc.date.accessioned2023-07-17T22:04:54Z
dc.date.available2023-07-17T22:04:54Z
dc.identifierLeón-Galván, F., de Jesús Joaquín-Ramos, A., Torres-Pacheco, I., Barba de la Rosa, A. P., Guevara-Olvera, L., González-Chavira, M. M., … Guevara-González, R. G. (2011). A Germin-Like Protein Gene (CchGLP) of Capsicum chinense Jacq. Is Induced during Incompatible Interactions and Displays Mn-Superoxide Dismutase Activity. International Journal of Molecular Sciences, 12(11), 7301–7313.
dc.identifierhttp://hdl.handle.net/11627/3699
dc.identifierhttp://doi.org/10.3390/ijms12117301
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7544164
dc.description"A germin-like gene (CchGLP) cloned from geminivirus-resistant pepper (Capsicum chinense Jacq. Line BG-3821) was characterized and the enzymatic activity of the expressed protein analyzed. The predicted protein consists of 203 amino acids, similar to other germin-like proteins. A highly conserved cupin domain and typical germin boxes, one of them containing three histidines and one glutamate, are also present in CchGLP. A signal peptide was predicted in the first 18 N-terminal amino acids, as well as one putative N-glycosylation site from residues 44-47. CchGLP was expressed in E. coli and the recombinant protein displayed manganese superoxide dismutase (Mn-SOD) activity. Molecular analysis showed that CchGLP is present in one copy in the C. chinense Jacq. genome and was induced in plants by ethylene (Et) and salicylic acid (SA) but not jasmonic acid (JA) applications in the absence of pathogens. Meanwhile, incompatible interactions with either Pepper golden mosaic virus (PepGMV) or Pepper huasteco yellow vein virus (PHYVV) caused local and systemic CchGLP induction in these geminivirus-resistant plants, but not in a susceptible accession. Compatible interactions with PHYVV, PepGMV and oomycete Phytophthora capsici did not induce CchGLP expression. Thus, these results indicate that CchGLP encodes a Mn-SOD, which is induced in the C. chinense geminivirus-resistant line BG-3821, likely using SA and Et signaling pathways during incompatible interactions with geminiviruses PepGMV and PHYVV"
dc.formatapplication/pdf
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsAcceso Abierto
dc.subjectGLP
dc.subjectGeminiviruses
dc.subjectMn-SOD
dc.subjectEthylene
dc.subjectSalicylic acid
dc.subjectBIOLOGÍA Y QUÍMICA
dc.titleA germin-like protein gene (CchGLP) of Capsicum chinense Jacq. is induced during incompatible interactions and displays Mn-superoxide dismutase activity
dc.typearticle


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