Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta

dc.creatorPaz Maldonado, Luz María Teresita
dc.creatorBalderas Hernández, Víctor Emmanuel
dc.creatorMedina Rivero, Emilio
dc.creatorBarba de la Rosa, Ana Paulina
dc.creatorFlores Flores, José Luis
dc.creatorOrdoñez Acevedo, Leandro Gabriel
dc.creatorDe León Rodríguez, Antonio
dc.date2018-06-07T20:16:37Z
dc.date2018-06-07T20:16:37Z
dc.date2007-06
dc.date.accessioned2023-07-17T22:03:30Z
dc.date.available2023-07-17T22:03:30Z
dc.identifierLuz M.T. Paz Maldonado, Víctor E. Balderas Hernández, Emilio Medina Rivero, Ana P. Barba de la Rosa, José L. Flores Flores, Leandro G. Ordoñez Acevedo, Antonio De León Rodríguez, Optimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: The case of human interferon beta, Biomolecular Engineering, Volume 24, Issue 2, 2007, Pages 217-222.
dc.identifierhttp://hdl.handle.net/11627/3841
dc.identifierhttps://doi.org/10.1016/j.bioeng.2006.10.001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7543536
dc.description"A human interferon beta (hINF-β) synthetic gene was optimized and expressed in Escherichia coli BL21-SI using a vector with the T7 promoter. To determine the best culture conditions such as culture medium, temperature, cell density and inducer concentration, we used the response surface methodology and a Box-Behnken design to get the highest hINF-β production. The maximum hINF-β production of 61 mg l−1 was attained using minimum medium and the following predicted optimal conditions: temperature of 32.5 °C, cell density of 0.64, and inducer concentration of 0.30 M NaCl. This is the first report showing the successful performance of the BL21-SI system in a minimum medium. The response surface methodology is effective for the optimization of recombinant protein production using synthetic genes."
dc.formatapplication/pdf
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsAcceso Abierto
dc.subjectCodon bias
dc.subjectE. coli BL21-SI
dc.subjectMultiple sclerosis
dc.subjectResponse surface methodology (RSM)
dc.subjectSynthetic gene
dc.subjectYeast extract
dc.subjectBIOLOGÍA MOLECULAR
dc.titleOptimization of culture conditions for a synthetic gene expression in Escherichia coli using response surface methodology: the case of human interferon beta
dc.typearticle


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